LGALS3 Mouse
Description
Product Specs
Product Science Overview
Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is a member of the galectin family of proteins. These proteins are characterized by their ability to bind specifically to β-galactosides through a conserved carbohydrate recognition domain (CRD). Galectin-3 is unique among galectins due to its chimeric structure, which includes one CRD linked to a non-lectin domain .
Galectin-3 is a multifunctional protein involved in various cellular processes, including cell adhesion, cell activation, chemoattraction, cell growth and differentiation, cell cycle regulation, and apoptosis . The protein’s ability to bind to β-galactosides is crucial for its role in these processes. The CRD of galectin-3 consists of approximately 130 amino acids, enabling specific binding to β-galactosides .
Recombinant mouse galectin-3 is typically produced using E. coli or HEK293 cells as expression systems . The recombinant protein is purified to a high degree of purity, often exceeding 95%, as determined by SDS-PAGE and visualized with silver staining or Coomassie Blue staining . The endotoxin level is kept below 1.0 EU per microgram of protein, ensuring its suitability for various experimental applications .
Recombinant mouse galectin-3 is widely used in research to study its role in various biological processes. It has been shown to agglutinate human red blood cells, with an effective dose (ED50) ranging from 5 to 20 µg/mL . Additionally, it supports the adhesion of mouse helper T cells, with an ED50 of 1-5 µg/mL . These properties make recombinant galectin-3 a valuable tool for studying cell-cell interactions, immune responses, and other cellular functions.
Recombinant mouse galectin-3 is typically lyophilized from a filtered solution containing various stabilizing agents such as PBS, EDTA, and DTT . The lyophilized protein should be reconstituted at a concentration of 200-250 µg/mL in sterile PBS or water, depending on the specific formulation . The protein is stable for up to 12 months when stored at -20 to -70°C and for up to 3 months at 2 to 8°C under sterile conditions after reconstitution .
