LGALS3 Human, His

Galectin-3 Human Recombinant, His Tag
Cat. No.
BT10794
Source
Escherichia Coli.
Synonyms
Galectin-3, GAL3, MAC2, CBP35, GALB, GALIG, LGALS2, LGALS3, Galactose-specific lectin 3, Mac-2 antigen, IgE-binding protein, 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Laminin-binding protein, Lectin L-29, L-31, Galactoside-binding protein, GALBP.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

LGALS3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 270 amino acids (1-250 a.a.) and having a molecular mass of 28.3 kDa. The LGALS3 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Galectin-3, in conjunction with the alpha-3, beta-1 integrin, facilitates cspg4-mediated stimulation of endothelial cell migration. It plays a crucial role in the development of vasculogenic mimicry and angiogenic properties associated with melanoma progression. LGALS3 exhibits high expression levels in the early stages of papillary carcinoma, with a decline in expression intensity as the tumor progresses. Elevated serum levels of LGALS3 are observed in patients with thyroid malignancy, although there is significant overlap in serum LGALS3 concentrations between individuals with benign and malignant nodular thyroid disease. Functioning as an immune regulator, LGLAS3 suppresses T-cell immune responses and promotes tumor growth, thereby providing a novel mechanism for tumor immune tolerance.
Description
Recombinant Human LGALS3, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 270 amino acids (1-250 a.a.) with a molecular weight of 28.3 kDa. The protein is fused to a 20 amino acid His-Tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The Galectin-3 protein solution is provided at a concentration of 0.5mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.1M NaCl, 1mM DTT, and 10% Glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product should be stored at 4°C. For longer storage periods, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, the addition of a carrier protein such as HSA or BSA (0.1%) is advised. Avoid subjecting the product to repeated freeze-thaw cycles.
Purity
The purity of the protein is determined to be greater than 95.0% using SDS-PAGE analysis.
Biological Activity
The biological activity of the protein is assessed by its ability to agglutinate human red blood cells. The ED50 for this effect is determined to be less than 2.5ug/ml.
Synonyms
Galectin-3, GAL3, MAC2, CBP35, GALB, GALIG, LGALS2, LGALS3, Galactose-specific lectin 3, Mac-2 antigen, IgE-binding protein, 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Laminin-binding protein, Lectin L-29, L-31, Galactoside-binding protein, GALBP.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MADNFSLHDA LSGSGNPNPQ GWPGAWGNQP AGAGGYPGAS YPGAYPGQAP PGAYPGQAPP GAYPGAPGAY PGAPAPGVYP GPPSGPGAYP SSGQPSATGA YPATGPYGAP AGPLIVPYNL PLPGGVVPRM LITILGTVKP NANRIALDFQ RGNDVAFHFN PRFNENNRRV IVCNTKLDNN WGREERQSVF PFESGKPFKI QVLVEPDHFK VAVNDAHLLQ YNHRVKKLNE ISKLGISGDI DLTSASYTMI.

Product Science Overview

Structure and Expression

Galectin-3 is a 31-kDa protein that contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain, which allows it to form oligomers . It is widely expressed in many normal tissues and a variety of tumors . The recombinant form of Galectin-3, tagged with a polyhistidine (His) tag, is typically expressed in Escherichia coli cells .

Biological Functions

Galectin-3 is involved in several biological processes, including:

  • Cell adhesion: It mediates cell-cell and cell-matrix interactions.
  • Cell activation and chemoattraction: It plays a role in the activation and attraction of immune cells.
  • Cell growth and differentiation: It is involved in the regulation of cell growth and differentiation.
  • Cell cycle and apoptosis: It has roles in cell cycle regulation and programmed cell death .

In the nucleus, Galectin-3 acts as a pre-mRNA splicing factor . It is also involved in acute inflammatory responses, including neutrophil activation and adhesion, chemoattraction of monocytes and macrophages, opsonization of apoptotic neutrophils, and activation of mast cells .

Clinical Relevance

Galectin-3 has been implicated in various diseases, including cancer, heart disease, and inflammatory conditions. Its role in cancer progression is particularly notable, as it is involved in tumor cell adhesion, migration, and angiogenesis . Additionally, Galectin-3 is a potential biomarker for heart failure and other cardiovascular diseases .

Applications

Recombinant Human Galectin-3 (His Tag) is used in various research applications, including:

  • SDS-PAGE: For protein analysis and characterization.
  • Cell-based assays: To study its biological functions and interactions.
  • Drug development: As a target for therapeutic interventions in diseases where Galectin-3 is implicated .

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