LGALS2 Human

Galectin-2 Human Recombinant
Cat. No.
BT10463
Source
Escherichia Coli.
Synonyms
HL14, Gal-2, Beta-galactoside-binding lectin L-14-II, Lactose-binding lectin 2, S-Lac lectin 2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

LGALS2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 152 amino acids (1-132 a.a.) and having a molecular mass of 16.8 kDa. The LGALS2 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
LGALS2, a soluble beta-galactoside binding lectin, regulates cell-to-cell and cell-to-extracellular matrix interactions. It plays a role in tumor development, pre-mRNA splicing, and apoptosis. LGALS2 can trigger apoptosis in activated T cells and binds to lymphotoxin-alpha (LTA), potentially increasing the risk of myocardial infarction.
Description
Recombinant Human LGALS2, expressed in E. coli, is a non-glycosylated polypeptide chain with 152 amino acids (1-132 a.a.) and a molecular weight of 16.8 kDa. A 20 amino acid His-Tag is fused to the N-terminus. Purification is achieved using proprietary chromatographic methods.
Physical Appearance
A sterile, colorless solution.
Formulation
The Galectin-2 His tag protein solution is provided at a concentration of 0.5mg/ml and contains 20mM Tris-HCl (pH 8), 0.2M NaCl, 10% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 95.0%, as assessed by SDS-PAGE.
Synonyms
HL14, Gal-2, Beta-galactoside-binding lectin L-14-II, Lactose-binding lectin 2, S-Lac lectin 2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MTGELEVKNM DMKPGSTLKI TGSIADGTDG FVINLGQGTD KLNLHFNPRF SESTIVCNSL DGSNWGQEQR
EDHLCFSPGS EVKFTVTFES DKFKVKLPDG HELTFPNRLG HSHLSYLSVR GGFNMSSFKL KE.

Product Science Overview

Introduction

Galectin-2 is a member of the galectin family, which consists of carbohydrate-binding proteins with a high affinity for β-galactosides. These proteins play crucial roles in various biological processes, including cell-cell adhesion, cell-matrix interactions, and immune responses. Galectin-2, in particular, has garnered interest due to its unique properties and potential therapeutic applications.

Structure and Expression

Galectin-2 is a homodimeric protein with a molecular weight of approximately 14 kDa . It shares structural similarities with galectin-1 but has a distinct expression profile. Unlike galectin-1, which is widely expressed in various tissues, galectin-2 is primarily confined to the gastrointestinal tract . This restricted expression pattern suggests specialized functions in this region.

Gene and Protein Characteristics

The gene encoding galectin-2 is located on chromosome 22 in humans. The protein consists of a single carbohydrate recognition domain (CRD), which is responsible for its binding to β-galactosides . The amino acid sequence of human galectin-2 shares significant identity with its mouse and rat counterparts, indicating a conserved function across species .

Biological Functions

Galectin-2 has been shown to play a role in various biological processes:

  1. Cell Adhesion and Migration: Galectin-2 can enhance the adhesion of human umbilical vein endothelial cells (HUVECs), suggesting a role in vascular biology .
  2. Immune Regulation: Galectin-2 induces apoptosis in activated T cells through a caspase-dependent pathway. This proapoptotic effect is mediated by the intrinsic apoptotic pathway, involving the release of cytochrome c and the regulation of Bax/Bcl-2 expression . This property positions galectin-2 as a potential therapeutic agent for modulating immune responses.
  3. Gastrointestinal Functions: Given its expression in the gastrointestinal tract, galectin-2 may be involved in maintaining gut homeostasis and modulating inflammatory responses in this region .
Recombinant Production

Recombinant human galectin-2 is typically produced using human embryonic kidney (HEK) 293 cells. The recombinant protein is purified to high levels of purity, often exceeding 95%, and is free from endotoxins . This high-quality recombinant protein is used in various research applications, including studies on cell adhesion, apoptosis, and immune regulation.

Therapeutic Potential

The unique properties of galectin-2, particularly its ability to induce apoptosis in activated T cells, make it a promising candidate for therapeutic applications. It could be explored as a treatment for autoimmune diseases, where the selective elimination of activated T cells is desirable. Additionally, its role in modulating immune responses opens avenues for its use in cancer immunotherapy and inflammatory diseases .

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Cat. No.
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Source
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THE BIOTEK
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