LDLRAP1 Human

Low Density Lipoprotein Receptor Adaptor Protein 1 Human Recombinant
Cat. No.
BT29524
Source
E.coli.
Synonyms
Low density lipoprotein receptor adaptor protein 1, Autosomal recessive hypercholesterolemia protein, ARH1, FHCB1, FHCB2, ARH2, LDL receptor adaptor protein, MGC34705, DKFZp586D0624.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

LDLRAP1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 328 amino acids (1-308) and having a molecular mass of 36.1kDa.
LDLRAP1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The LDLRAP1 protein is found in the cytoplasm and plays a crucial role in binding to a specific sequence in proteins called the phosphotyrosine binding (PTD) domain. It functions as an adaptor molecule within the cell, connecting the LDLR (Low-Density Lipoprotein Receptor) to the machinery responsible for endocytosis, the process of bringing molecules into the cell. Mutations in the LDLRAP1 gene can lead to a condition known as autosomal recessive hypercholesterolemia (ARH). This condition arises from the inability of the liver to effectively internalize LDL receptors (LDLR), resulting in elevated cholesterol levels in the blood.
Description
Recombinant LDLRAP1, produced in E.coli, is a single polypeptide chain that is not glycosylated. It consists of 328 amino acids, specifically amino acids 1 to 308, and has a molecular weight of 36.1 kDa. For purification purposes, a 20 amino acid His-tag is attached to the N-terminus of the protein. The protein is further purified using proprietary chromatographic techniques.
Physical Appearance
The solution is sterile, filtered, and colorless.
Formulation
The LDLRAP1 solution has a concentration of 0.5 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 200 mM NaCl, 2 mM DTT, and 10% glycerol.
Stability
For optimal storage, refrigerate the solution at 4°C if the entire vial will be used within 2-4 weeks. For extended storage, freeze the solution at -20°C. To ensure long-term stability, it is recommended to add a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing should be avoided.
Purity
Analysis by SDS-PAGE indicates that the purity of the protein is greater than 90%.
Synonyms
Low density lipoprotein receptor adaptor protein 1, Autosomal recessive hypercholesterolemia protein, ARH1, FHCB1, FHCB2, ARH2, LDL receptor adaptor protein, MGC34705, DKFZp586D0624.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MDALKSAGRA LIRSPSLAKQ SWGGGGRHRK LPENWTDTRE TLLEGMLFSL KYLGMTLVEQ PKGEELSAAA IKRIVATAKA SGKKLQKVTL KVSPRGIILT DNLTNQLIEN VSIYRISYCT ADKMHDKVFA YIAQSQHNQS LECHAFLCTK RKMAQAVTLT VAQAFKVAFE FWQVSKEEKE KRDKASQEGG DVLGARQDCT PPLKSLVATG NLLDLEETAK APLSTVSANT TNMDEVPRPQ ALSGSSVVWE LDDGLDEAFS RLAQSRTNPQ VLDTGLTAQD MHYAQCLSPV DWDKPDSSGT EQDDLFSF

Product Science Overview

Structure and Function

LDLRAP1 contains a phosphotyrosine binding (PTB) domain that interacts with the cytoplasmic tail of the LDL receptor . This interaction is essential for the efficient internalization of LDLR-LDL complexes from coated pits, which are specialized regions of the plasma membrane involved in receptor-mediated endocytosis . The protein binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface, thereby stabilizing the interaction between the receptor and the structural components of the pits .

Genetic Implications

Mutations in the LDLRAP1 gene can lead to autosomal recessive hypercholesterolemia (ARH), a disorder characterized by elevated levels of low-density lipoprotein cholesterol (LDL-C) in the blood . This condition increases the risk of cardiovascular diseases, such as atherosclerosis and coronary artery disease . The malfunction of LDLR due to these mutations impairs the body’s ability to remove LDL-C from the bloodstream, leading to its accumulation .

Clinical Significance

The study of LDLRAP1 is significant for understanding and potentially treating hypercholesterolemia and related cardiovascular diseases . By exploring the mechanisms of LDLRAP1 and its interactions with LDLR, researchers aim to develop targeted therapies that can enhance the clearance of LDL-C from the bloodstream .

Research and Applications

Human recombinant LDLRAP1 is used in research to study its role in cholesterol metabolism and its potential therapeutic applications . Recombinant proteins are produced through genetic engineering techniques, allowing scientists to investigate the protein’s structure, function, and interactions in a controlled environment .

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