LCN2 Human, His
Single band on Western Blot.
Description
The LCN2 is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
NGAL was initially discovered in activated neutrophils, hence its name. It is also expressed in other tissues, including the kidney, liver, and lungs. The protein has a characteristic lipocalin fold, which consists of an eight-stranded antiparallel β-barrel. This structure allows NGAL to bind to siderophores, small molecules that chelate iron, which is essential for bacterial growth .
NGAL is involved in the innate immune response by sequestering iron and preventing its use by bacteria, thus limiting their growth . It binds to bacterial siderophores as well as the mammalian siderophore 2,5-dihydroxybenzoic acid (2,5-DHBA), ensuring that excess free iron does not accumulate in the cytoplasm . This function is crucial in preventing bacterial infections and maintaining iron homeostasis.
NGAL is a promising biomarker for acute kidney injury (AKI). Injured epithelial cells in the kidney secrete a monomeric form of NGAL, while activated neutrophils secrete a dimeric form . NGAL levels in blood and urine rise significantly within two hours of kidney injury, making it a more sensitive and earlier marker for AKI compared to traditional markers like serum creatinine . Additionally, NGAL has been associated with chronic kidney disease, contrast-induced nephropathy, and kidney transplant outcomes .
Recombinant human NGAL is produced using recombinant DNA technology, where the NGAL gene is cloned and expressed in a suitable host system. The His tag, a sequence of histidine residues, is added to facilitate purification of the protein using affinity chromatography. This recombinant form is used in various research and clinical applications to study the protein’s function and its role in disease processes .
NGAL has been extensively studied for its role in kidney diseases, cancer, and inflammation. It is used as a biomarker in clinical settings to diagnose and monitor kidney injury and other conditions. Research continues to explore its potential therapeutic applications, including its role in drug delivery and as a target for novel treatments .
