LCN1 Human
Lipocalin-1 Human Recombinant
Cat. No.
BT6069
Source
Escherichia Coli.
Synonyms
Lipocalin-1, PMFA, TLC, TP, VEGP, Tear lipocalin, Tear prealbumin, Von Ebner gland protein, VEG protein.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
LCN1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 183 amino acids (19-176 a.a) and having a molecular mass of 20.1kDa.
LCN1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
LCN1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Lipocalin-1 (LCN1), a member of the lipocalin family, is a small secreted protein. Lipocalins function as extracellular transporters, binding to a variety of hydrophobic ligands. As the primary lipid-binding protein in tears, LCN1 is upregulated in response to various stimuli, including infection and stress. Additionally, LCN1 serves as a marker for chromosome aneuploidy and as an autoantigen in Sjogren's syndrome.
Description
Recombinant human LCN1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 183 amino acids (residues 19-176). With a molecular weight of 20.1 kDa, LCN1 is expressed with a 25 amino acid His-tag fused to its N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, colorless solution that has been filtered for clarity.
Formulation
The LCN1 protein solution is provided at a concentration of 1 mg/ml and is formulated in phosphate-buffered saline (pH 7.4) containing 10% glycerol and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product should be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To enhance long-term stability during freezing, the addition of a carrier protein (0.1% HSA or BSA) is advised. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the LCN1 protein is determined to be greater than 90.0% using SDS-PAGE analysis.
Synonyms
Lipocalin-1, PMFA, TLC, TP, VEGP, Tear lipocalin, Tear prealbumin, Von Ebner gland protein, VEG protein.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMHHLLA SDEEIQDVSG TWYLKAMTVD REFPEMNLES VTPMTLTTLE
GGNLEAKVTM LISGRCQEVK AVLEKTDEPG KYTADGGKHV AYIIRSHVKD HYIFYCEGEL HGKPVRGVKL
VGRDPKNNLE ALEDFEKAAG ARGLSTESIL IPRQSETCSP GSD.
Product Science Overview
Gene and Protein Structure
Lipocalin-1 is encoded by the LCN1 gene . The protein is produced by the lacrimal glands and secreted into the tear fluid, where it plays a crucial role in maintaining the health and stability of the ocular surface . The recombinant form of Lipocalin-1 is typically produced in a laboratory setting using a mouse myeloma cell line (NS0) and is tagged with a C-terminal 10-His tag for purification purposes .
Function and Importance
Lipocalin-1 acts as a lipid sponge on the ocular surface, binding to various lipophilic substances and preventing them from causing damage to the eye . It has been shown to inhibit the activity of certain proteases, such as Cathepsin V, which can degrade extracellular matrix components and contribute to tissue damage .
Applications in Research and Medicine
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