LAMP1 Human
Sf9, Baculovirus cells.
Lysosome-associated membrane glycoprotein 1, LAMP1, CD107a, LAMPA, LGP120, LAMP-1, CD107 antigen-like family member A.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
LAMP1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 363 amino acids (29-382a.a.) and having a molecular mass of 39.4kDa. (Molecular size on SDS-PAGE will appear at approximately 57-70kDa).
LAMP1 is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Lysosomal-Associated Membrane Protein 1 (LAMP1), a member of the LAMP family, is a membrane protein found in the endosome-lysosome membranes of cells. It plays a role in tumor cell metastasis. A specific glycoform of LAMP1 is present on the surface of activated macrophages, where it contributes to T cell costimulation and promotes a Th1-biased immune response. LAMP1 is also observed on the plasma membrane during the activation of various immune cells, including NK cells, CD8+ T cells, monocytes, basophils, and platelets.
Produced in Sf9 Baculovirus cells, LAMP1 is a single, glycosylated polypeptide chain consisting of 363 amino acids (29-382a.a.). It has a molecular weight of 39.4kDa, although it appears between 57-70kDa on SDS-PAGE. This LAMP1 protein is expressed with a 9 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
The product is a sterile filtered solution, colorless in appearance.
The LAMP1 protein solution is provided at a concentration of 1mg/ml. It is formulated in phosphate buffered saline (pH 7.4) with 10% glycerol.
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it should be frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
The purity of LAMP1 is greater than 90.0% as determined by SDS-PAGE analysis.
Lysosome-associated membrane glycoprotein 1, LAMP1, CD107a, LAMPA, LGP120, LAMP-1, CD107 antigen-like family member A.
Sf9, Baculovirus cells.
ADPAMFMVKN GNGTACIMAN FSAAFSVNYD TKSGPKNMTF DLPSDATVVL NRSSCGKENT SDPSLVIAFG RGHTLTLNFT RNATRYSVQL MSFVYNLSDT HLFPNASSKE IKTVESITDI RADIDKKYRC VSGTQVHMNN VTVTLHDATI QAYLSNSSFS RGETRCEQDR PSPTTAPPAP
PSPSPSPVPK SPSVDKYNVS GTNGTCLLAS MGLQLNLTYE RKDNTTVTRL LNINPNKTSA SGSCGAHLVT LELHSEGTTV LLFQFGMNAS SSRFFLQGIQ LNTILPDARD PAFKAANGSL RALQATVGNS YKCNAEEHVR VTKAFSVNIF KVWVQAFKVE GGQFGSVEEC LLDENSMHHH
HHH
Product Science Overview
LAMP1 is a highly glycosylated protein with a polypeptide core of approximately 40 kDa. It consists of a large luminal domain, a single transmembrane domain, and a short cytoplasmic tail. The luminal domain contains multiple N-glycosylation sites, which are essential for the addition of sugar chains that protect the protein from degradation by lysosomal proteases .
LAMP1 is primarily located on the lysosomal membrane and is involved in various cellular processes, including:
- Lysosomal Biogenesis: LAMP1 plays a role in the formation and maintenance of lysosomes, which are essential for cellular waste disposal and recycling.
- pH Regulation: It helps regulate the acidic environment within lysosomes, which is necessary for the optimal activity of lysosomal enzymes .
- Autophagy: LAMP1 is involved in the autophagic process, where cells degrade and recycle their own components .
- Cholesterol Homeostasis: It plays a role in maintaining cholesterol balance within cells .
- Immune Response: LAMP1 is a marker of degranulation in immune cells such as CD8+ T cells and natural killer (NK) cells. It is involved in the movement of cytotoxic granules to the cell surface and the trafficking of perforin to lytic granules .
LAMP1 has been implicated in various diseases and conditions:
- Cancer: LAMP1 expression is associated with tumor cell invasion and metastasis. It is found in the cytoplasm of several types of tumor cells and may play a role in tumor progression .
- Immune Disorders: Abnormal LAMP1 expression is linked to immune-related conditions such as hemophagocytic lymphohistiocytosis .
- Infectious Diseases: LAMP1 acts as a receptor for certain pathogens, including the Lassa virus .
Recombinant LAMP1 is produced using recombinant DNA technology, which involves inserting the LAMP1 gene into a suitable expression system, such as bacteria or mammalian cells. This allows for the large-scale production of LAMP1 protein for research and therapeutic purposes. Recombinant LAMP1 is used in various applications, including:
- Antibody Production: It is used to generate antibodies for research and diagnostic purposes.
- Functional Studies: Recombinant LAMP1 is used to study its role in lysosomal function, immune response, and disease mechanisms.
- Therapeutic Development: It is being explored as a potential therapeutic target for diseases such as cancer and immune disorders .
