LAIR2 Human

Leukocyte-Associated Ig-Like Receptor 2 Human Recombinant
Cat. No.
BT2753
Source
Escherichia Coli.
Synonyms
Leukocyte-associated immunoglobulin-like receptor 2, LAIR-2, CD306, LAIR2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

LAIR2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 139 amino acids (22-135 a.a) and having a molecular mass of 15.2kDa.
LAIR2 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Leukocyte-associated immunoglobulin-like receptor 2 (LAIR2) is a secreted protein composed of 131 amino acids. Classified as a member of the Ig superfamily due to its single Ig-like C2 type domain, LAIR2 is believed to have a common gene ancestor with LAIR1, an inhibitory receptor found on mononuclear leukocytes. Despite their shared evolutionary history and potential for similar adhesion profiles, LAIR2 is secreted while LAIR1 remains membrane-bound. This suggests a possible competitive relationship for ligand binding between the two. Discovered through its resemblance to LAIR1, LAIR2's gene is located within the leukocyte receptor cluster at 19q13.4, a region home to 29 immunoglobulin superfamily genes, including LAIR1. Although the precise function of LAIR2 remains unclear, its secreted nature suggests a potential role in modulating mucosal tolerance.
Description
Recombinant human LAIR2, produced in E. coli, is a non-glycosylated polypeptide chain consisting of 139 amino acids (specifically, amino acids 22-135). This protein has a molecular weight of 15.2 kDa and features a 25 amino acid His-tag at its N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The LAIR2 protein solution is provided at a concentration of 1 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 20% glycerol, 0.1 M NaCl, and 1 mM DTT.
Stability
For short-term storage (up to 4 weeks), the LAIR2 protein solution should be kept at 4°C. For long-term storage, it is recommended to freeze the solution at -20°C. To ensure optimal stability during long-term storage, adding a carrier protein such as HSA or BSA (0.1%) is advised. Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of LAIR2 is determined by SDS-PAGE analysis and is consistently greater than 90%.
Synonyms
Leukocyte-associated immunoglobulin-like receptor 2, LAIR-2, CD306, LAIR2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMQEGAL PRPSISAEPG TVISPGSHVT FMCRGPVGVQ TFRLEREDRA KYKDSYNVFR LGPSESEARF HIDSVSEGNA GLYRCLYYKP PGWSEHSDFL ELLVKGTVPG TEASGFDAP.

Product Science Overview

Structure and Expression

LAIR-2 is a soluble receptor that shares 84% sequence homology with LAIR-1, another member of the LAIR family . Unlike LAIR-1, which is a membrane-bound receptor, LAIR-2 lacks a transmembrane and cytoplasmic region, indicating that it is secreted . The protein has a single immunoglobulin (Ig)-like domain and is expressed in various tissues, including peripheral blood mononuclear cells (PBMCs) .

Function

LAIR-2 functions as a natural competitor to LAIR-1 by binding to collagen molecules with high affinity . This binding is hydroxyproline-dependent, and LAIR-2 can inhibit the interaction between LAIR-1 and collagen . By doing so, LAIR-2 regulates the inhibitory potential of LAIR-1, which is known to inhibit immune cell function upon collagen binding . This regulatory mechanism is crucial for maintaining immune homeostasis and preventing excessive immune reactions or autoimmune diseases .

Clinical Significance

The expression of LAIR-2 has been observed in various clinical conditions. For instance, high levels of LAIR-2 have been detected in the urine of pregnant women and in the synovial fluid of patients with rheumatoid arthritis . These findings suggest that LAIR-2 may play a role in immune regulation during pregnancy and in inflammatory diseases .

Research and Applications

Research on LAIR-2 has provided insights into its potential therapeutic applications. By understanding the mechanisms through which LAIR-2 regulates immune responses, scientists can develop strategies to modulate its activity for therapeutic purposes. For example, targeting LAIR-2 could be beneficial in treating autoimmune diseases or in enhancing immune responses against infections and tumors .

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Source
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