L1CAM Human
Sf9, Baculovirus cells.
L1 Cell Adhesion Molecule, Antigen Identified By Monoclonal Antibody R1, N-CAM-L1, NCAM-L1, CAML1, MIC5, Neural Cell Adhesion Molecule L1, CD171 Antigen, N-CAML1, CD171, HSAS1, MASA, HSAS, SPG1, S10.
Greater than 90.0% as determined by SDS-PAGE.
Description
L1CAM Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 1104 amino acids (20-1115a.a.) and having a molecular mass of 123.6kDa (Molecular size on SDS-PAGE will appear at approximately 100-150kDa).
L1CAM is expressed with a 8 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
The L1 Cell Adhesion Molecule (L1CAM) is a cell adhesion receptor belonging to the immunoglobulin superfamily. It plays a crucial role in nerve cell function, specifically in neural cell adhesion. This molecule is involved in the dynamic regulation of cell adhesion and the generation of transmembrane signals at tyrosine kinase receptors. L1CAM is also vital for cell migration, neurite outgrowth, and myelination. Moreover, it contributes significantly to the dynamic regulation of neuronal structure and function in the mature brain.
Recombinant human L1CAM, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It comprises 1104 amino acids (20-1115a.a.) and has a molecular mass of 123.6kDa. It's important to note that the molecular size on SDS-PAGE might appear between 100-150kDa. This L1CAM protein is expressed with an 8 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
The L1CAM protein solution is provided at a concentration of 0.25mg/ml. It is formulated in Phosphate Buffered Saline (pH 7.4) and contains 10% glycerol.
The purity of the L1CAM protein is greater than 90.0% as determined by SDS-PAGE analysis.
The biological activity of the L1CAM protein is determined by its ability to support Neuro-2a mouse neuroblastoma cell adhesion. The immobilized protein exhibits more than 30% activity when cells are added to human L1CAM coated plates at a concentration of 1 ug/ml.
L1 Cell Adhesion Molecule, Antigen Identified By Monoclonal Antibody R1, N-CAM-L1, NCAM-L1, CAML1, MIC5, Neural Cell Adhesion Molecule L1, CD171 Antigen, N-CAML1, CD171, HSAS1, MASA, HSAS, SPG1, S10.
Sf9, Baculovirus cells.
IQIPEELMEP PVITEQSPRR LVVFPTDDIS LKCEASGKPE VQFRWTRDGV HFKPKEELGV TVYQSPHSGS FTITGNNSNF AQRFQGIYRC FASNKLGTAM SHEIRLMAEG APKWPKETVK PVEVEEGESV VLPCNPPPSA EPLRIYWMNS KILHIKQDER VTMGQNGNLY FANVLTSDNH SDYICHAHFP GTRTIIQKEP IDLRVKATNS MIDRKPRLLF PTNSSSHLVA LQGQPLVLEC IAEGFPTPTI KWLRPSGPMP ADRVTYQNHN KTLQLLKVGE EDDGEYRCLA ENSLGSARHA YYVTVEAAPY WLHKPQSHLY GPGETARLDC QVQGRPQPEV TWRINGIPVE ELAKDQKYRI QRGALILSNV QPSDTMVTQC EARNRHGLLL ANAYIYVVQL PAKILTADNQ TYMAVQGSTA YLLCKAFGAP VPSVQWLDED GTTVLQDERF FPYANGTLGI RDLQANDTGR YFCLAANDQN NVTIMANLKV KDATQITQGP RSTIEKKGSR VTFTCQASFD PSLQPSITWR GDGRDLQELG DSDKYFIEDG RLVIHSLDYS DQGNYSCVAS TELDVVESRA QLLVVGSPGP VPRLVLSDLH LLTQSQVRVS WSPAEDHNAP IEKYDIEFED KEMAPEKWYS LGKVPGNQTS TTLKLSPYVH YTFRVTAINK YGPGEPSPVS ETVVTPEAAP EKNPVDVKGE GNETTNMVIT WKPLRWMDWN APQVQYRVQW RPQGTRGPWQ EQIVSDPFLV VSNTSTFVPY EIKVQAVNSQ GKGPEPQVTI GYSGEDYPQA IPELEGIEIL NSSAVLVKWR PVDLAQVKGH LRGYNVTYWR EGSQRKHSKR HIHKDHVVVP ANTTSVILSG LRPYSSYHLE VQAFNGRGSG PASEFTFSTP EGVPGHPEAL HLECQSNTSL LLRWQPPLSH NGVLTGYVLS YHPLDEGGKG QLSFNLRDPE LRTHNLTDLS PHLRYRFQLQ ATTKEGPGEA IVREGGTMAL SGISDFGNIS ATAGENYSVV SWVPKEGQCN FRFHILFKAL GEEKGGASLS PQYVSYNQSS YTQWDLQPDT DYEIHLFKER MFRHQMAVKT NGTGRVRLPP AGFATELEHH HHHH.
Product Science Overview
L1CAM is encoded by the L1CAM gene located on the X chromosome (Xq28) in humans . The protein has a molecular weight of approximately 200-220 kDa and consists of six immunoglobulin-like domains and five fibronectin type III repeats . The extracellular domain of L1CAM is responsible for its adhesive properties, while the intracellular domain interacts with the cytoskeleton to influence cell signaling and migration .
L1CAM is predominantly expressed in the nervous system, where it is found on the surface of neurons, Schwann cells, and other glial cells . It is involved in various biological processes, including:
- Cell Migration: L1CAM facilitates the migration of neurons during development and regeneration .
- Neurite Outgrowth: It promotes the extension of neurites, which are essential for the formation of neural networks .
- Myelination: L1CAM plays a role in the myelination of axons, which is critical for efficient nerve signal transmission .
- Cell Adhesion: It mediates homophilic (L1CAM-L1CAM) and heterophilic (L1CAM-other molecules) cell adhesion, contributing to the structural integrity of neural tissues .
Mutations in the L1CAM gene are associated with a group of disorders known as L1 syndrome, which includes conditions such as X-linked hydrocephalus, MASA syndrome (Mental retardation, Aphasia, Shuffling gait, and Adducted thumbs), and spastic paraplegia . These disorders are characterized by neurological deficits due to impaired neuronal migration and connectivity .
L1CAM is also implicated in cancer progression and metastasis. It is overexpressed in various malignancies, including gastric, ovarian, and colorectal cancers . High levels of L1CAM expression are associated with poor prognosis and increased metastatic potential . Inhibition of L1CAM has been shown to reduce tumor cell proliferation, invasion, and migration, making it a potential therapeutic target .
Human recombinant L1CAM is produced using recombinant DNA technology, which involves inserting the L1CAM gene into a suitable expression system, such as bacteria or mammalian cells. This allows for the large-scale production of the protein for research and therapeutic purposes. Recombinant L1CAM is used in various studies to understand its function, interactions, and potential as a therapeutic target in neurological disorders and cancer .
