KRT5 Antibody
Description
Product Specs
Product Science Overview
Cytokeratin 5 (CK5) is a type of intermediate filament protein that is part of the cytoskeleton in epithelial cells. It plays a crucial role in maintaining the structural integrity and mechanical resilience of epithelial tissues. The mouse anti-human Cytokeratin 5 antibody is a monoclonal antibody specifically designed to detect human CK5 in various research applications.
Cytokeratins are a subfamily of intermediate filament proteins characterized by their remarkable biochemical diversity. They are expressed in epithelial tissues and are involved in various cellular functions, including structural support, cellular signaling, and differentiation. CK5, in particular, is a type II cytokeratin that pairs with type I cytokeratin 14 (CK14) to form intermediate filaments in the basal layer of stratified epithelia .
CK5 is a valuable biomarker in cancer research, especially in the study of epithelial malignancies. Its expression patterns are used to distinguish different types of epithelial cancers, such as squamous cell carcinoma and basal cell carcinoma. Additionally, CK5 is used in the classification of breast cancer subtypes, particularly in identifying basal-like breast cancers .
The mouse anti-human CK5 antibody is a monoclonal antibody that specifically binds to human CK5. It is commonly used in various immunological techniques, including immunohistochemistry (IHC), flow cytometry, and immunofluorescence (IF). This antibody is highly specific and has been validated for use in multiple applications, making it a reliable tool for researchers .
- Immunohistochemistry (IHC): The mouse anti-human CK5 antibody is widely used in IHC to detect CK5 expression in tissue samples. This technique is essential for studying the distribution and localization of CK5 in different tissues and for identifying CK5-positive cells in cancerous tissues .
- Flow Cytometry: This antibody is also used in flow cytometry to analyze CK5 expression in cell populations. Flow cytometry allows for the quantitative measurement of CK5 levels in individual cells, providing valuable insights into cellular heterogeneity and the presence of CK5-positive cells in mixed populations .
- Immunofluorescence (IF): In IF, the mouse anti-human CK5 antibody is used to visualize CK5 expression in cells using fluorescently labeled secondary antibodies. This technique enables the study of CK5 distribution within cells and the observation of CK5 co-localization with other cellular components .
