KRT14 Antibody
Cytokeratin 14, Mouse Anti Human
Cat. No.
BT22801
Source
Synonyms
Keratin, type I cytoskeletal 14, Cytokeratin-14, CK-14,
Keratin-14, K14, KRT14, NFJ, CK14, EBS3, EBS4.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
Product Specs
Introduction
Cytokeratin 14, a type I keratin, belongs to the keratin family, the most diverse group of intermediate filaments. It typically forms a heterotetramer with two keratin 5 molecules, a type II keratin. This complex constitutes the cytoskeleton of epithelial cells. Notably, mutations in the genes encoding these keratins are linked to epidermolysis bullosa simplex. A pseudogene has been mapped to chromosome 17p12-p11.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The antibody is supplied at a concentration of 1 mg/ml in a solution of phosphate-buffered saline (PBS) at pH 7.4, supplemented with 10% glycerol and 0.02% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), maintain the antibody at 4°C. For extended storage, store at -20°C. Repeated freezing and thawing should be avoided.
Stability / Shelf Life
The antibody remains stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This antibody has been validated for use in ELISA and Western blot analysis, ensuring its specificity and reactivity. However, as with all antibodies, the optimal working dilution may vary depending on the specific application. It is recommended to perform a titration with a starting dilution of 1:1000.
Synonyms
Keratin, type I cytoskeletal 14, Cytokeratin-14, CK-14,
Keratin-14, K14, KRT14, NFJ, CK14, EBS3, EBS4.
Purification Method
KRT14 antibody was purified from mouse ascitic fluids by
protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT13F4AT.
Immunogen
Anti-human KRT14 mAb,
clone PAT13F4AT, is derived from hybridization of mouse F0 myeloma cells with
spleen cells from BALB/c mice immunized with a recombinant human KRT14 protein 1-472
amino acids purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and k light chain.
Product Science Overview
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