KLRD1 Antibody
Killer Cell Lectin Like Receptor D1, Killer Cell Lectin-Like Receptor Subfamily D, Member 1, NK Cell Receptor, CD94 Antigen, CD94, KP43, Killer Cell Lectin-Like Receptor Subfamily D Member 1, Natural Killer Cells Antigen CD94, KLRD1.
Description
Product Specs
KLRD1, also known as Killer Cell Lectin Like Receptor D1, is a protein found on the surface of natural killer cells, which are part of the innate immune system. This protein acts like a sensor, allowing NK cells to recognize and bind to another molecule called HLA-E, which is often found on the surface of other cells. KLRD1 can pair with proteins from the NKG2 family to form a larger complex. This complex, which may include CD94, plays a crucial role in the ability of NK cells to identify and potentially eliminate target cells.
The antibody is supplied in a solution containing 1 milligram of antibody per milliliter. The solution is buffered to a pH of 7.4 using PBS and contains 10% glycerol and 0.02% sodium azide as preservatives.
This KLRD1 antibody has undergone rigorous testing using ELISA and Western blot techniques, confirming its specificity and reactivity. Optimal working concentrations may vary depending on the specific application and should be determined empirically.
Killer Cell Lectin Like Receptor D1, Killer Cell Lectin-Like Receptor Subfamily D, Member 1, NK Cell Receptor, CD94 Antigen, CD94, KP43, Killer Cell Lectin-Like Receptor Subfamily D Member 1, Natural Killer Cells Antigen CD94, KLRD1.
KLRD1 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT13E3AT.
Anti-human KLRD1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human KLRD1 protein 32-279 amino acids purified from E. coli.
Mouse IgG1 heavy chain and k light chain.
Product Science Overview
Killer cell lectin-like receptor D1 (KLRD1), also known as CD94, is a protein-coding gene that plays a crucial role in the immune system. It is predominantly expressed on the surface of natural killer (NK) cells and certain subsets of T-cells. KLRD1 is involved in the regulation of immune responses, particularly in the recognition and elimination of infected or malignant cells .
KLRD1 is a type II transmembrane protein characterized by an extracellular C-type lectin-like domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic tail . The extracellular domain is responsible for binding to its ligands, which include human leukocyte antigen (HLA) class I molecules. This interaction is crucial for the modulation of NK cell activity.
The primary function of KLRD1 is to provide inhibitory signals that regulate the activation and proliferation of NK cells and T-cells. By binding to HLA class I molecules, KLRD1 helps to prevent the destruction of normal, healthy cells, thereby maintaining immune homeostasis .
The production of mouse anti-human KLRD1 antibodies involves several steps:
- Immunization: Mice are immunized with purified human KLRD1 protein or peptides derived from the KLRD1 sequence. This process stimulates the mouse immune system to produce antibodies against KLRD1.
- Hybridoma Technology: Spleen cells from the immunized mice are fused with myeloma cells to create hybridoma cells. These hybridoma cells are capable of producing monoclonal antibodies specific to human KLRD1.
- Screening and Selection: The hybridoma cells are screened for the production of high-affinity antibodies against KLRD1. Positive clones are selected and expanded for further use.
- Purification: The monoclonal antibodies are purified from the culture supernatant using techniques such as protein A/G affinity chromatography.
Mouse anti-human KLRD1 antibodies are valuable tools in immunological research and clinical diagnostics. They are commonly used in:
- Flow Cytometry: To analyze the expression of KLRD1 on the surface of NK cells and T-cells.
- Immunohistochemistry: To detect KLRD1 in tissue samples.
- Functional Assays: To study the role of KLRD1 in immune cell regulation and its involvement in various diseases.
KLRD1 has been implicated in several diseases, including cancers, autoimmune disorders, and infectious diseases. Its expression patterns and functional roles in these conditions make it a potential biomarker and therapeutic target . For instance, alterations in KLRD1 expression have been associated with the progression of lung adenocarcinoma and the response to immunotherapy .
