KIR3DL2 Human
Killer Cell Immunoglobulin Like Receptor, Three Ig Domains And Long Cytoplasmic Tail 2, Killer Cell Immunoglobulin-Like Receptor, Three Domains, Long Cytoplasmic Tail, 2, P70 Natural Killer Cell Receptor Clone CL-5, Natural Killer-Associated Transcript 4, CD158 Antigen-Like Family Member K, MHC Class I NK Cell Receptor, P70 NK Receptor CL-5, CD158K, NKAT-4, NKAT4, Killer Cell Immunoglobulin-Like Receptor 3DL2, Killer-Cell Immunoglobulin-Like Receptor, P70 Killer Cell Inhibitory Receptor, Killer Ig Receptor, KIR Antigen 3DL2, CD158k Antigen, KIR-3DL2, NKAT4B, 3DL2, P140.
Greater than 85.0% as determined by SDS-PAGE.
Description
KIR3DL2 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 561 amino acids (22-340a.a.) and having a molecular mass of 62.2kDa (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).
KIR3DL2 is expressed with a 239 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Killer Cell Immunoglobulin Like Receptor, Three Ig Domains And Long Cytoplasmic Tail 2, Killer Cell Immunoglobulin-Like Receptor, Three Domains, Long Cytoplasmic Tail, 2, P70 Natural Killer Cell Receptor Clone CL-5, Natural Killer-Associated Transcript 4, CD158 Antigen-Like Family Member K, MHC Class I NK Cell Receptor, P70 NK Receptor CL-5, CD158K, NKAT-4, NKAT4, Killer Cell Immunoglobulin-Like Receptor 3DL2, Killer-Cell Immunoglobulin-Like Receptor, P70 Killer Cell Inhibitory Receptor, Killer Ig Receptor, KIR Antigen 3DL2, CD158k Antigen, KIR-3DL2, NKAT4B, 3DL2, P140.
ADPLMGGQDK PFLSARPSTV VPRGGHVALQ CHYRRGFNNF MLYKEDRSHV PIFHGRIFQE SFIMGPVTPA HAGTYRCRGS RPHSLTGWSA PSNPLVIMVT GNHRKPSLLA HPGPLLKSGE TVILQCWSDV MFEHFFLHRE GISEDPSRLV GQIHDGVSKA NFSIGPLMPV LAGTYRCYGS VPHSPYQLSA PSDPLDIVIT GLYEKPSLSA QPGPTVQAGE NVTLSCSSWS SYDIYHLSRE GEAHERRLRA VPKVNRTFQA DFPLGPATHG GTYRCFGSFR ALPCVWSNSS DPLLVSVTGN PSSSWPSPTE PSSKSGICRH LHVEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGKHHHHH H.
Product Science Overview
Killer Cell Immunoglobulin-Like Receptors (KIRs) are a family of receptors expressed on the surface of natural killer (NK) cells and some T-cell subsets. These receptors play a crucial role in the regulation of the immune response by interacting with major histocompatibility complex (MHC) class I molecules. Among these receptors, the Killer Cell Immunoglobulin-Like Receptor, 3 Domains Long Cytoplasmic Tail 2 (KIR3DL2) is of particular interest due to its unique structure and function.
KIR3DL2 is characterized by its three immunoglobulin-like domains, designated as D0, D1, and D2 . These domains are sequentially arranged and contribute to the receptor’s ability to bind to its ligands. The receptor also includes a signal peptide, a stem region, a transmembrane region, and a long cytoplasmic tail . The cytoplasmic tail contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs), which are essential for transmitting inhibitory signals within the cell .
The KIR3DL2 gene is located on chromosome 19q13.4 and is part of the KIR gene cluster . This gene cluster is known for its high degree of polymorphism, which contributes to the diversity of the immune response among individuals. KIR3DL2 is expressed primarily on NK cells and some T-cell subsets . The expression of KIR3DL2 can be regulated by various factors, including DNA methylation and the presence of specific transcription factors .
KIR3DL2 plays a critical role in the immune system by regulating the activity of NK cells. It interacts with MHC class I molecules on the surface of target cells, transmitting inhibitory signals that prevent the NK cells from killing normal, healthy cells . This mechanism is essential for maintaining self-tolerance and preventing autoimmune reactions. Additionally, KIR3DL2 has been implicated in the recognition and elimination of infected or transformed cells, contributing to the body’s defense against infections and cancer .
Recombinant KIR3DL2 is produced using the Sf9 insect cell expression system. This system is commonly used for the production of recombinant proteins due to its ability to perform post-translational modifications similar to those in mammalian cells. The recombinant KIR3DL2 protein retains the functional properties of the native receptor, making it a valuable tool for research and therapeutic applications.
Recombinant KIR3DL2 has several applications in biomedical research and clinical practice. It can be used to study the interactions between KIRs and MHC class I molecules, providing insights into the mechanisms of immune regulation. Additionally, recombinant KIR3DL2 can be employed in the development of novel immunotherapies for cancer and infectious diseases. By modulating the activity of NK cells, these therapies have the potential to enhance the immune response against tumors and pathogens.
