KIR2DL5A Antibody
Killer Cell Immunoglobulin Like Receptor, Two Ig Domains And Long Cytoplasmic Tail 5A, Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail 5A, KIR2DL5, CD158F, Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail, 5, Killer Cell Immunoglobulin-Like Receptor KIR2DL5A, Killer Cell Immunoglobulin-Like Receptor 2DL5A, CD158f1 Antigen, KIR2DL5.1, KIR2DL5.3, CD158F1, KIR2DL5A.
Description
Product Specs
Killer cell immunoglobulin-like receptor 2DL5A, also known as KIR2DL5A, belongs to the killer cell Ig-like receptor (KIR) family. It is a type I transmembrane glycoprotein found on the cell membrane as a monomer. Upon tyrosine phosphorylation, KIR2DL5A recruits the Src homology region 2-containing protein tyrosine phosphatase-2. As an inhibitory receptor with a distinct combination of genetic, structural, and functional characteristics within the KIR family, KIR2DL5A is believed to play a vital role in innate immunity.
The antibody solution has a concentration of 1 mg/ml and is prepared in a buffer containing PBS with a pH of 7.4, 10% glycerol, and 0.02% sodium azide.
The KIR2DL5A antibody has been validated for use in ELISA, Western blot analysis, and ICC/IF to ensure its specificity and reactivity. However, as applications may vary, it is recommended to titrate the antibody for each experiment to achieve optimal results.
Killer Cell Immunoglobulin Like Receptor, Two Ig Domains And Long Cytoplasmic Tail 5A, Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail 5A, KIR2DL5, CD158F, Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail, 5, Killer Cell Immunoglobulin-Like Receptor KIR2DL5A, Killer Cell Immunoglobulin-Like Receptor 2DL5A, CD158f1 Antigen, KIR2DL5.1, KIR2DL5.3, CD158F1, KIR2DL5A.
KIR2DL5A antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT11C11AT.
Anti-human KIR2DL5A mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human KIR2DL5A amino acids 22-238 purified from E. coli.
Mouse IgG2b heavy chain and κ light chain.
Product Science Overview
Killer Cell Immunoglobulin-Like Receptors (KIRs) are a family of receptors expressed on the surface of natural killer (NK) cells and some T cells. These receptors play a crucial role in the regulation of the immune response by recognizing specific molecules on the surface of target cells. KIRs can be either activating or inhibitory, and their interaction with ligands on target cells determines the activation or inhibition of NK cell cytotoxicity.
KIR2DL5A is a member of the KIR family, characterized by having two immunoglobulin-like domains and a long cytoplasmic tail. The “2DL” in its name indicates the presence of two extracellular immunoglobulin-like domains and a long cytoplasmic tail. The long cytoplasmic tail contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs), which are responsible for transmitting inhibitory signals to the NK cell upon ligand binding .
The extracellular region of KIR2DL5A contains two C2-type Ig-like domains, which are involved in ligand recognition. The transmembrane region is hydrophobic, allowing the receptor to anchor in the cell membrane. The cytoplasmic tail contains two ITIMs, which recruit intracellular protein tyrosine phosphatases such as SHP-1 upon phosphorylation. This recruitment leads to the inhibition of NK cell activation and cytotoxicity .
KIR2DL5A recognizes specific human leukocyte antigen (HLA) class I molecules as its ligands. The interaction between KIR2DL5A and its ligands on target cells results in the transmission of inhibitory signals, preventing the NK cell from killing the target cell. This mechanism is essential for maintaining self-tolerance and preventing autoimmunity .
The KIR gene family is located on chromosome 19q13.4 and exhibits a high degree of polymorphism. This polymorphism contributes to the diversity of NK cell responses among individuals. KIR2DL5A is one of the inhibitory KIRs, and its gene is closely related to other KIR genes, such as KIR2DL1 and KIR2DL2 .
The study of KIR2DL5A and other KIRs is important for understanding the regulation of NK cell activity in various clinical contexts, including transplantation, infectious diseases, and cancer. The expression of KIR2DL5A and its interaction with HLA class I molecules can influence the outcome of hematopoietic stem cell transplantation and the progression of certain viral infections and tumors .
Mouse anti-human KIR2DL5A antibodies are used in research to study the expression and function of KIR2DL5A on human NK cells. These antibodies can be used in various applications, including flow cytometry, immunoprecipitation, and functional assays, to investigate the role of KIR2DL5A in immune regulation .
