KCTD4 Human

KCTD4 is a protein-coding gene that produces a protein involved in various cellular processes. The human recombinant version of KCTD4 is produced in E. coli as a single, non-glycosylated polypeptide chain containing 282 amino acids, with a molecular mass of 32.4 kDa . This recombinant protein is fused to a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques .

The KCTD4 protein contains a BTB/POZ domain, which is known for its role in protein-protein interactions and transcriptional regulation through the control of chromatin structure and function . The BTB domain is involved in homodimerization, which is essential for the protein’s function .

KCTD4 is involved in several biological pathways, including sweet taste signaling and the activation of cAMP-dependent protein kinase A (PKA) . These pathways are crucial for various cellular functions, including signal transduction and metabolic regulation.

The recombinant human KCTD4 protein is used in various research applications, including studies on protein-protein interactions, transcriptional regulation, and potassium channel function. It is also used in the development of assays and as a tool for understanding the molecular mechanisms underlying various diseases.

The recombinant KCTD4 protein is typically stored at 4°C if it will be used within 2-4 weeks. For longer storage, it is recommended to store the protein frozen at -20°C with a carrier protein to prevent freeze-thaw cycles .