HARS Human, Sf9
Histidyl-tRNA Synthetase Human Recombinant, Sf9
Cat. No.
BT26683
Source
Sf9 Insect Cells.
Synonyms
Histidyl-tRNA synthetase, EC 6.1.1.21, Histidine-tRNA ligase, HisRS, HRS, FLJ20491, JO-1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Histidyl-tRNA Synthetase Human Recombinant produced in baculovirus is a single, glycosylated, polypeptide chain having a molecular mass of 58.3 kDa.
The Histidyl-tRNA Synthetase is fused to 6x His Tag and purified by proprietary chromatographic techniques.
The Histidyl-tRNA Synthetase is fused to 6x His Tag and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Aminoacyl-tRNA synthetases are enzymes that link tRNAs to their corresponding amino acids. This gene encodes a cytoplasmic class II aminoacyl-tRNA synthetase. This enzyme creates histidyl-transfer RNA, which is necessary for incorporating histidine into proteins. The gene shares a bidirectional promoter with HARSL on chromosome five and is positioned head-to-head with it. In the human autoimmune illness polymyositis/dermatomyositis, the gene product is frequently the target of autoantibodies.
Description
Baculovirus-produced Histidyl-tRNA Synthetase Human Recombinant is a single glycosylated polypeptide chain with a molecular weight of 58.3 kDa. Histidyl-tRNA Synthetase is fused with a 6x His Tag and purified using specialized chromatographic methods.
Physical Appearance
Clear, sterile filtered solution.
Formulation
The protein solution contains 20mM HEPES, 250mM sodium chloride, 0.1% and 20% Glycerol, at pH 7.5.
Stability
Although Histidyl-tRNA Synthetase is stable at 4°C for three weeks, it is recommended to store it at a temperature below -18°C to ensure optimal preservation. Avoid repeated freeze-thaw cycles.
Purity
SDS-PAGE analysis indicates a purity greater than 90.0%.
Protein Content
Using a Bradford assay with BSA as a standard, protein quantification was performed at concentrations ranging from 0.25 to 2.0 mg/ml.
Applications
Western Blot: Exhibits strong reactivity with human anti-Histidyl-tRNA Synthetase antisera.
Synonyms
Histidyl-tRNA synthetase, EC 6.1.1.21, Histidine-tRNA ligase, HisRS, HRS, FLJ20491, JO-1.
Source
Sf9 Insect Cells.
Product Science Overview
Structure and Function
Histidyl-tRNA Synthetase is a cytoplasmic enzyme that plays a pivotal role in the synthesis of histidyl-transfer RNA (tRNA). This enzyme ensures the incorporation of the amino acid histidine into proteins during translation. The human recombinant version of this enzyme, produced in Sf9 insect cells, is a single, glycosylated polypeptide chain with a molecular mass of approximately 58.3 kDa .
Production and Purification
The recombinant HARS is produced using baculovirus expression systems in Sf9 insect cells. This method allows for the production of a glycosylated form of the enzyme, which is then purified using proprietary chromatographic techniques. The enzyme is fused to a 6x His Tag to facilitate purification .
Stability and Storage
Applications
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