Introduction
Histidyl-tRNA synthetase (HARS) is an enzyme that facilitates the attachment of the amino acid histidine to its corresponding tRNA molecule. This process, known as aminoacylation, is essential for protein synthesis. HARS belongs to the class II family of aminoacyl-tRNA synthetases. It plays a crucial role in ensuring the accurate incorporation of histidine into proteins. Autoantibodies targeting HARS are frequently observed in individuals with polymyositis/dermatomyositis, an autoimmune disease.
Description
This product consists of the recombinant human HARS protein, expressed in E. coli and purified to a high degree. A 23-amino acid His tag is fused to the N-terminus to facilitate purification. The protein is a single, non-glycosylated polypeptide chain with a molecular weight of 59.4 kDa. It encompasses amino acids 1-509 of the full HARS sequence. Purification is achieved using proprietary chromatographic methods, resulting in a highly pure protein.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The HARS protein is supplied in a solution containing 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1mM DTT, and 0.1M NaCl. The protein concentration is 1 mg/ml.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To further enhance stability during long-term storage, adding a carrier protein like HSA or BSA to a final concentration of 0.1% is advisable. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the HARS protein is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
Histidyl-tRNA synthetase cytoplasmic, Histidine--tRNA ligase, HisRS, HARS, HRS, FLJ20491.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAERAAL EELVKLQGER VRGLKQQKAS AELIEEEVAK LLKLKAQLGP DESKQKFVLK TPKGTRDYSP RQMAVREKVF DVIIRCFKRH GAEVIDTPVF ELKETLMGKY GEDSKLIYDL KDQGGELLSL RYDLTVPFAR YLAMNKLTNI KRYHIAKVYR RDNPAMTRGR YREFYQCDFD IAGNFDPMIP DAECLKIMCE ILSSLQIGDF LVKVNDRRIL DGMFAICGVS DSKFRTICSS VDKLDKVSWE EVKNEMVGEK GLAPEVADRI GDYVQQHGGV SLVEQLLQDP KLSQNKQALE GLGDLKLLFE YLTLFGIDDK ISFDLSLARG LDYYTGVIYE AVLLQTPAQA GEEPLGVGSV AAGGRYDGLV GMFDPKGRKV PCVGLSIGVE RIFSIVEQRL EALEEKIRTT ETQVLVASAQ KKLLEERLKL VSELWDAGIK AELLYKKNPK LLNQLQYCEE AGIPLVAIIG EQELKDGVIK LRSVTSREEV DVRREDLVEE IKRRTGQPLC IC.
