ITGB1 Human, Sf9
Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29, ITGB1, FNRB, MDF2, MSK12, Integrin beta 1, CD29, VLAB.
Greater than 95.0% as determined by SDS-PAGE.
Description
ITGB1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 716 amino acids (1-728) and having a molecular mass of 79.4kDa (Molecular size on SDS-PAGE will appear at approximately 70-100kDa). ITGB1 is fused to an 8 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29, ITGB1, FNRB, MDF2, MSK12, Integrin beta 1, CD29, VLAB.
QTDENRCLKA NAKSCGECIQ AGPNCGWCTN STFLQEGMPT SARCDDLEAL KKKGCPPDDI ENPRGSKDIK KNKNVTNRSK GTAEKLKPED ITQIQPQQLV LRLRSGEPQT FTLKFKRAED YPIDLYYLMD LSYSMKDDLE NVKSLGTDLM NEMRRITSDF RIGFGSFVEK TVMPYISTTP AKLRNPCTSE QNCTSPFSYK NVLSLTNKGE VFNELVGKQR ISGNLDSPEG GFDAIMQVAV CGSLIGWRNV TRLLVFSTDA GFHFAGDGKL GGIVLPNDGQ CHLENNMYTM SHYYDYPSIA HLVQKLSENN IQTIFAVTEE FQPVYKELKN LIPKSAVGTL SANSSNVIQL IIDAYNSLSS EVILENGKLS EGVTISYKSY CKNGVNGTGE NGRKCSNISI GDEVQFEISI TSNKCPKKDS DSFKIRPLGF TEEVEVILQY ICECECQSEG IPESPKCHEG NGTFECGACR CNEGRVGRHC ECSTDEVNSE DMDAYCRKEN SSEICSNNGE CVCGQCVCRK RDNTNEIYSG KFCECDNFNCDRSNGLICGG NGVCKCRVCE CNPNYTGSAC DCSLDTSTCE ASNGQICNGR GICECGVCKC TDPKFQGQTC EMCQTCLGVC AEHKECVQCR AFNKGEKKDT CTQECSYFNI TKVESRDKLP QPVQPDPVSH CKEKDVDDCW FYFTYSVNGN NEVMVHVVEN PECPTGPDLE HHHHHH
Product Science Overview
Integrin Beta 1, also known as CD29 or ITGB1, is a cell surface receptor that plays a crucial role in cell adhesion and recognition processes. It is encoded by the ITGB1 gene in humans . Integrins are heterodimeric proteins composed of alpha and beta subunits, and Integrin Beta 1 can pair with various alpha subunits to form different integrin complexes .
Integrin Beta 1 exists in multiple isoforms due to alternative splicing. These isoforms include Beta-1A, Beta-1B, Beta-1C, and Beta-1D, each with distinct functions and tissue distributions . The protein has a large extracellular domain, a single transmembrane segment, and a short cytoplasmic tail that interacts with the actin cytoskeleton .
Integrin Beta 1 is involved in various cellular processes, including:
- Cell Adhesion: It mediates the attachment of cells to the extracellular matrix (ECM) and other cells .
- Signal Transduction: Integrin Beta 1 transmits signals from the ECM to the cell interior, influencing cell behavior such as migration, proliferation, and survival .
- Tissue Repair and Immune Response: It plays a role in wound healing and immune cell trafficking .
Recombinant Integrin Beta 1 is produced using the Sf9 insect cell expression system. Sf9 cells, derived from the fall armyworm (Spodoptera frugiperda), are commonly used for the production of recombinant proteins due to their high expression levels and ability to perform post-translational modifications .
Recombinant Integrin Beta 1 is used in various research applications, including:
- Functional Assays: To study cell adhesion, migration, and signal transduction pathways .
- Drug Development: As a target for developing therapeutics for diseases involving abnormal cell adhesion and migration, such as cancer and fibrosis .
- Structural Studies: To understand the molecular mechanisms of integrin function and regulation .
