ITGB1 Antibody
Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29, ITGB1, FNRB, MDF2, MSK12, Integrin beta 1, CD29, VLAB.
Description
Product Specs
Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29, ITGB1, FNRB, MDF2, MSK12, Integrin beta 1, CD29, VLAB.
ITGB1 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT47E2AT.
Anti-human ITGB1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ITGB1 protein 21-461 amino acids purified from E. coli.
Mouse IgG1 heavy chain and k light chain.
Product Science Overview
Integrins are a family of cell surface receptors that play a crucial role in cell adhesion, migration, and signal transduction. They are heterodimeric proteins composed of alpha and beta subunits. Among these, integrin beta 1 (ITGB1) is a significant subunit that pairs with various alpha subunits to form different integrin receptors. The mouse anti-human integrin beta 1 antibody is a monoclonal antibody used extensively in research to study the function and distribution of integrin beta 1 in human tissues.
Integrin beta 1 is a transmembrane protein that interacts with extracellular matrix (ECM) components such as collagen, fibronectin, and laminin. It consists of a large extracellular domain, a single transmembrane domain, and a short cytoplasmic tail. The extracellular domain is responsible for binding to ECM ligands, while the cytoplasmic tail interacts with intracellular signaling molecules .
Integrin beta 1 is involved in various cellular processes, including:
- Cell adhesion: It mediates the attachment of cells to the ECM, which is essential for tissue integrity and repair.
- Cell migration: It plays a role in the movement of cells during development, wound healing, and immune responses.
- Signal transduction: It transmits signals from the ECM to the cell interior, influencing cell survival, proliferation, and differentiation .
The mouse anti-human integrin beta 1 antibody is typically produced by immunizing mice with a synthetic peptide corresponding to a specific region of the human integrin beta 1 protein. The immune response generates antibodies that specifically recognize and bind to human integrin beta 1. These antibodies are then harvested from the mice and purified using techniques such as protein A affinity chromatography .
The mouse anti-human integrin beta 1 antibody is widely used in various research applications, including:
- Western blotting: To detect integrin beta 1 protein levels in cell and tissue lysates.
- Immunohistochemistry: To visualize the distribution of integrin beta 1 in tissue sections.
- Flow cytometry: To analyze the expression of integrin beta 1 on the surface of cells.
- Immunoprecipitation: To isolate integrin beta 1 protein complexes from cell lysates for further analysis .
Integrin beta 1 has been implicated in several diseases, including cancer, fibrosis, and inflammatory conditions. In cancer, integrin beta 1 is involved in tumor cell adhesion, migration, and invasion, contributing to metastasis. It also plays a role in the resistance of cancer cells to chemotherapy and radiotherapy . Therefore, the mouse anti-human integrin beta 1 antibody is a valuable tool for studying the mechanisms underlying these diseases and developing potential therapeutic strategies.
