ISG15 Antibody
Interferon stimulated gene 15, Mouse Anti Human
Cat. No.
BT21365
Source
Synonyms
ISG15, G1P2, IFI15, UCRP, Interferon-induced 17 kDa protein precursor, ISG15 Ubiquitin-like modifier.
Appearance
Sterile Filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
ISG15, an ubiquitin-like modifier, is conjugated to intracellular target proteins upon stimulation by IFN-alpha or IFN-beta. The enzymatic pathway of ISG15 is similar to that of ubiquitin but exhibits differences in substrate specificity and interaction with ligating enzymes. While ISG15 conjugation utilizes a dedicated E1 enzyme, it appears to share the same E2 enzyme with the ubiquitin conjugation pathway. Targets of ISG15 protein include STAT1, SERPINA 3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MXA, and RIG-1. Deconjugation of ISG15 by USP18/UBP43 triggers chemotactic activity in neutrophils, leading to the release of eosinophil chemotactic factors. Acting as a trans-acting binding factor, ISG15 directs the association of ligated target proteins with intermediate filaments. ISG15 participates in autocrine, paracrine, and endocrine mechanisms, potentially influencing cell-to-cell signaling by inducing IFN-gamma secretion from monocytes and macrophages.
Physical Appearance
A clear, colorless solution that is sterile-filtered.
Formulation
The solution contains 1mg/ml of ISG15 antibody in a buffer of PBS at pH 7.4 with 0.1% sodium azide.
Storage Procedures
For storage up to 1 month, keep at 4°C. For longer storage, keep at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The product is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This ISG15 antibody has been validated for ELISA and Western blot applications, ensuring its specificity and reactivity. However, optimal working dilutions should be determined empirically for each application. For Western blot analysis, a dilution range of 1:1,000 to 1:2,000 is recommended, with a starting dilution of 1:1,000.
Synonyms
ISG15, G1P2, IFI15, UCRP, Interferon-induced 17 kDa protein precursor, ISG15 Ubiquitin-like modifier.
Purification Method
ISG15 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
P3E5AT.
Immunogen
Anti-human ISG15 mAb is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human ISG15 amino acids 1-157 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and κ light chain.
Product Science Overview
Mouse Anti-Human Antibodies
Mouse anti-human antibodies are secondary antibodies generated by immunizing mice with human immunoglobulins . These antibodies are affinity-purified and have well-characterized specificity for human immunoglobulins . They are commonly used in various detection systems, such as HRP, AP, and fluorescence, and provide greater sensitivity through signal amplification .
Applications of Mouse Anti-Human Antibodies
Mouse anti-human antibodies are used in:
- Detection: They are used to detect human proteins in various assays, including ELISA, Western blot, and immunohistochemistry .
- Sorting and Purification: These antibodies help in sorting and purifying human proteins from complex mixtures .
- Research: They are essential tools in biomedical research for studying human proteins and their interactions .
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