IRF1 Human

IFN Regulatory Factor-1 Human Recombinant
Cat. No.
BT28773
Source
Escherichia Coli.
Synonyms
IRF-1, IRF1, MAR.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

IRF1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids (1-114) with a His Tag of 20 aa, and having a molecular mass of 15 kDa.
The IRF1 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
IRF1, short for IFN regulatory factor 1, plays a crucial role in regulating gene expression vital for immune response, hematopoiesis, and proliferation. As a transcription factor, IRF1 activates genes stimulated by IFN-A, IFN-B, and IFN-G. Additionally, it acts as a tumor suppressor, triggering apoptosis in tumorigenic cell lines.
Description
Recombinant human IRF1, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 134 amino acids (residues 1-114). It includes a 20-amino acid His tag and has a molecular weight of 15 kDa. The purification of IRF1 is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The product is supplied at a concentration of 1 mg/ml in a buffer consisting of 20mM Tris (pH 8) and 10% glycerol.
Stability
Liquid IRF1 remains stable at 10°C for up to one week; however, it is recommended to store it below -18°C for optimal long-term stability. Adding a carrier protein like 0.1% HSA or BSA is advised for extended storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 90%, as determined by reverse-phase high-performance liquid chromatography (RP-HPLC) and sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
Synonyms
IRF-1, IRF1, MAR.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MPITRMRMRP WLEMQINSNQ IPGLIWINKE EMIFQIPWKHAAKHGWDINK DACLFRSWAI HTGRYKAGEK EPDPKTWKAN FRCAMNSLPD IEEVKDQSRN KGSSAVRVYR MLPP.

Product Science Overview

Introduction

Interferon Regulatory Factor-1 (IRF-1) is a crucial transcription factor in the regulation of interferon (IFN) genes and plays a significant role in the immune response. It is a member of the IRF family, which consists of several transcription factors involved in the regulation of the IFN system and other genes related to immune responses .

Discovery and Structure

IRF-1 was first identified as a transcription factor that binds to the IFN-stimulated response element (ISRE) in the promoters of IFN and IFN-stimulated genes . The IRF-1 protein comprises 325 amino acids and contains an N-terminal DNA-binding domain (DBD) characterized by a helix-turn-helix motif. This motif is highly conserved among all IRF proteins and is responsible for recognizing specific DNA sequences .

Functions and Mechanisms

IRF-1 is known for its role in various cellular processes, including:

  • Immune Response: IRF-1 activates the transcription of IFN-α and IFN-β genes in response to viral infections .
  • Tumor Suppression: It acts as a tumor suppressor by regulating genes involved in cell cycle control, apoptosis, and DNA damage response .
  • Cell Cycle Regulation: IRF-1 influences the expression of genes that control the cell cycle, thereby playing a role in cell proliferation and differentiation .
Recombinant IRF-1

Recombinant human IRF-1 is produced using genetic engineering techniques, typically in Escherichia coli (E. coli) cells. The gene encoding IRF-1 is cloned and expressed in E. coli, resulting in the production of the recombinant protein . This recombinant protein is used in various research applications to study the biological functions of IRF-1 and its role in immune responses and tumor suppression .

Applications in Research

Recombinant IRF-1 is valuable in research for several reasons:

  • Studying Immune Responses: It helps in understanding how IRF-1 regulates the expression of IFN genes and other immune-related genes .
  • Cancer Research: Researchers use recombinant IRF-1 to investigate its role in tumor suppression and its potential as a therapeutic target .
  • Drug Development: The protein is used in screening assays to identify compounds that modulate IRF-1 activity, which could lead to the development of new therapies for viral infections and cancer .

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