IFN b Antibody
Description
Product Specs
Product Science Overview
Interferon-beta (IFN-β) is a type I interferon, a group of cytokines known for their antiviral activities and their role in modulating the immune system. IFN-β is produced by various cell types, including fibroblasts and macrophages, in response to viral infections and other stimuli. It plays a crucial role in the innate immune response by inhibiting viral replication and activating immune cells.
IFN-β is a monomeric glycoprotein composed of approximately 166 amino acids. It binds to the interferon-alpha/beta receptor (IFNAR), which is a heterodimer consisting of IFNAR1 and IFNAR2 subunits. Upon binding to its receptor, IFN-β triggers a signaling cascade that leads to the activation of various genes involved in antiviral defense, immune regulation, and cell proliferation .
IFN-β has been widely studied for its therapeutic potential in various diseases. One of its most notable applications is in the treatment of multiple sclerosis (MS), a chronic autoimmune disease that affects the central nervous system. IFN-β helps reduce the frequency and severity of MS relapses by modulating the immune response and reducing inflammation .
Mouse anti-human IFN-β antibodies are monoclonal antibodies developed in mice that specifically target human IFN-β. These antibodies are used in various research and diagnostic applications, including:
- Western Blotting: To detect and quantify IFN-β protein levels in biological samples.
- ELISA (Enzyme-Linked Immunosorbent Assay): To measure IFN-β concentrations in serum, plasma, or cell culture supernatants.
- Neutralization Assays: To study the biological activity of IFN-β and its role in immune responses.
- Immunohistochemistry: To visualize the distribution and localization of IFN-β in tissue samples .
The development of mouse anti-human IFN-β antibodies involves immunizing mice with human IFN-β protein or peptides, followed by the isolation and cloning of specific antibody-producing B cells. These antibodies are then characterized for their specificity, affinity, and functionality. They are essential tools in biomedical research, enabling scientists to study the role of IFN-β in various physiological and pathological processes .
