ING1 Human

Inhibitor of Growth Family, Member 1 Human Recombinant
Cat. No.
BT24375
Source
Escherichia Coli.
Synonyms
Inhibitor Of Growth Family, Member 1, Growth Inhibitory Protein ING1, Tumor Suppressor ING1, Growth Inhibitor ING1, Inhibitor Of Growth Protein 1, Inhibitor Of Growth 1, P24ING1c,P33ING1b, P47ING1a, P33ING1, P33, P47, ING1 .
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

ING1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 302 amino acids (1-279 a.a) and having a molecular mass of 34.3kDa.
ING1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
ING1, also known as Inhibitor of Growth Family Member 1, is a tumor suppressor protein that can halt cell growth and trigger apoptosis (programmed cell death). This nuclear protein directly interacts with the renowned tumor suppressor protein TP53 and plays a crucial role in the p53 signaling pathway, which regulates cell growth and death. Notably, decreased ING1 expression and structural alterations have been observed in various cancers, highlighting its significance in preventing tumor development. Furthermore, ING1 exhibits multiple isoforms due to alternative splicing, resulting in different protein forms with potentially diverse functions.
Description
Recombinant ING1 protein, produced in E. coli bacteria, is a single polypeptide chain that lacks glycosylation modifications. This protein comprises 302 amino acids, specifically residues 1 to 279, resulting in a molecular weight of 34.3 kDa. For purification and detection purposes, ING1 is tagged with a 23-amino acid His-tag at its N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The ING1 protein is provided as a solution at a concentration of 1 mg/ml. The solution is buffered with 20mM Tris-HCl at a pH of 8.0 and contains 10% glycerol as a stabilizing agent.
Stability
For short-term storage (2-4 weeks), keep refrigerated at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Avoid repeated freezing and thawing.
Purity
The purity of ING1 is greater than 90%, as assessed by SDS-PAGE analysis.
Synonyms
Inhibitor Of Growth Family, Member 1, Growth Inhibitory Protein ING1, Tumor Suppressor ING1, Growth Inhibitor ING1, Inhibitor Of Growth Protein 1, Inhibitor Of Growth 1, P24ING1c,P33ING1b, P47ING1a, P33ING1, P33, P47, ING1 .
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMLSPANG EQLHLVNYVE DYLDSIESLP FDLQRNVSLM REIDAKYQEI LKELDECYER FSRETDGAQK RRMLHCVQRA LIRSQELGDE KIQIVSQMVE LVENRTRQVD SHVELFEAQQ ELGDTAGNSG KAGADRPKGE AAAQADKPNS KRSRRQRNNE NRENASSNHD HDDGASGTPK EKKAKTSKKK KRSKAKAERE ASPADLPIDP NEPTYCLCNQ VSYGEMIGCD NDECPIEWFH FSCVGLNHKP KGKWYCPKCR GENEKTMDKA LEKSKKERAY NR.

Product Science Overview

Introduction

The Inhibitor of Growth (ING) family comprises a group of tumor suppressor proteins that play crucial roles in regulating cell growth, apoptosis, and cellular senescence. Among these, Inhibitor of Growth Family, Member 1 (ING1) is particularly significant due to its involvement in various cellular processes and its potential implications in cancer development and progression .

Structure and Function

ING1 is a nuclear protein that interacts with the tumor suppressor protein TP53 (p53) and is a component of the p53 signaling pathway . This interaction is vital for the regulation of cell growth and apoptosis. ING1 contains several domains that allow it to bind to chromatin structures and exert its effects as a reader of histone modification marks . These domains include:

  • Plant Homeodomain (PHD) Finger: This domain is responsible for recognizing and binding to specific histone modifications, which is crucial for the regulation of gene expression.
  • Leucine Zipper-like Domain: This domain facilitates protein-protein interactions, allowing ING1 to form complexes with other proteins involved in chromatin remodeling and transcriptional regulation.
Role in Cancer

ING1 acts as a tumor suppressor by inducing cell growth arrest and apoptosis . Its expression is often reduced or rearranged in various cancers, highlighting its importance in maintaining normal cellular functions. The loss of ING1 function can lead to uncontrolled cell proliferation and tumor development .

Mechanisms of Action

ING1 exerts its tumor suppressor functions through several mechanisms:

  1. Regulation of p53 Activity: ING1 enhances the transcriptional activity of p53, leading to the activation of genes involved in cell cycle arrest and apoptosis .
  2. Histone Modification: By binding to specific histone modifications, ING1 can influence chromatin structure and gene expression .
  3. Epigenetic Regulation: ING1 is involved in both histone acetylation and deacetylation complexes, playing a role in the epigenetic regulation of gene expression .
Clinical Implications

The study of ING1 and its role in cancer has significant clinical implications. Understanding the molecular mechanisms by which ING1 functions can lead to the development of new therapeutic strategies for cancer treatment. Additionally, the detection of ING1 expression levels could serve as a diagnostic marker for certain types of cancer .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.