IMPA2 Human
Inositol Monophosphatase 2 Human Recombinant
Cat. No.
BT29205
Source
Escherichia Coli.
Synonyms
Inositol monophosphatase 2, IMP 2, IMPase 2, Inositol-1(or 4)-monophosphatase 2, Myo-inositol monophosphatase A2, IMPA2, IMP.18P.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
IMPA2 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 308 amino acids (1-288 a.a.) and having a molecular mass of 33.5kDa. The IMPA2 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
IMPA2, a member of the inositol monophosphatase family, catalyzes the dephosphorylation of inositol monophosphate, playing a crucial role in phosphatidylinositol signaling. This enzyme utilizes various substrates, including myo-inositol monophosphates, scylloinositol 1,4-diphosphate, glucose-1-phosphate, beta-glycerophosphate, and 2'-AMP. IMPA2 serves as a pharmacological target for lithium (Li+) action in the brain and is believed to be involved in schizophrenia and bipolar disorder.
Description
Recombinant human IMPA2, fused with a 20 amino acid His tag at the N-terminus, is produced in E. coli. This single, non-glycosylated polypeptide chain comprises 308 amino acids (residues 1-288) and has a molecular weight of 33.5 kDa. Purification of IMPA2 is achieved through proprietary chromatographic techniques.
Physical Appearance
Sterile, colorless solution.
Formulation
The IMPA2 solution (0.25 mg/ml) is supplied in 20mM Tris-HCl buffer (pH 8.0), containing 10% glycerol and 2mM DTT.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 95.0% as determined by SDS-PAGE analysis.
Synonyms
Inositol monophosphatase 2, IMP 2, IMPase 2, Inositol-1(or 4)-monophosphatase 2, Myo-inositol monophosphatase A2, IMPA2, IMP.18P.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MKPSGEDQAA LAAGPWEECF QAAVQLALRA GQIIRKALTE EKRVSTKTSA ADLVTETDHL VEDLIISELR ERFPSHRFIA EEAAASGAKC VLTHSPTWII DPIDGTCNFV HRFPTVAVSI GFAVRQELEF GVIYHCTEER LYTGRRGRGA FCNGQRLRVS GETDLSKALV LTEIGPKRDP ATLKLFLSNM ERLLHAKAHG VRVIGSSTLA LCHLASGAAD AYYQFGLHCW DLAAATVIIR EAGGIVIDTS GGPLDLMACR VVAASTREMA MLIAQALQTI NYGRDDEK.
Product Science Overview
Introduction
Structure and Expression
Enzymatic Activity
IMPA2 demonstrates enzymatic activity by utilizing various substrates such as:
- Myo-inositol monophosphates
- Scylloinositol 1,4-diphosphate
- Glucose-1-phosphate
- Beta-glycerophosphate
- 2’-AMP
This enzyme is involved in the dephosphorylation of inositol monophosphates, which is a critical step in the phosphatidylinositol signaling pathway .
Biological Functions
IMPA2 is implicated in several biological functions, including:
- Regulation of intracellular inositol levels: It helps maintain the balance of inositol and its derivatives, which are vital for cell signaling.
- Neurological functions: Given its high expression in the brain, IMPA2 is believed to play a role in neurological processes and has been associated with psychiatric disorders such as bipolar disorder .
Pharmacological Target
Recombinant Production
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