IL 28B Mouse

Recombinant mouse IL-28B is typically produced in E. coli and is a single, non-glycosylated polypeptide chain containing 174 amino acids, with a molecular mass of approximately 19.6 kDa . The recombinant protein is purified using proprietary chromatographic techniques to ensure high purity and activity .

IL-28B exhibits several common features with type I interferons, including antiviral and antitumor activities . It plays a crucial role in the immune response to viral infections by inducing the expression of interferon-stimulated genes (ISGs) that inhibit viral replication. Additionally, IL-28B has been shown to have potential therapeutic applications in treating certain cancers due to its ability to modulate the immune response .

Recombinant mouse IL-28B is widely used in research to study its biological functions and potential therapeutic applications. It is often utilized in anti-viral assays to evaluate its efficacy in inhibiting viral replication in various cell lines . Moreover, it is used in studies investigating the role of IL-28B in immune modulation and its potential as a therapeutic agent in cancer treatment .

The recombinant protein is typically lyophilized from a filtered solution in PBS and can be reconstituted at a concentration of 100 μg/mL in sterile PBS . It is recommended to store the protein at -20 to -70 °C to maintain its stability and avoid repeated freeze-thaw cycles . Under sterile conditions, the reconstituted protein can be stored at 2 to 8 °C for up to one month or at -20 to -70 °C for up to three months .