IL28B Human
Interleukin 28B Human Recombinant
Cat. No.
BT12844
Source
Sf9, Baculovirus cells.
Synonyms
Lambda 3, IL28B, Interleukin 28B (IFN, Lambda 3), Cytokine Zcyto22, Interleukin-28B, Interleukin-28C, IFN-Lambda-3, IL-28B, IL-28C, IL28C, Lambda 4, Interleukin 28B, Interleukin 28C, IFN-Lambda-4, ZCYTO22, IFNL3.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
IL 28B produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (22-196 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 181 amino acids and having a molecular mass of 20.4kDa.
IL 28B shows multiple bands between 18-28kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
IL 28B shows multiple bands between 18-28kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
Product Specs
Introduction
IL-28B, belonging to the type I IFN family, shares a close relationship with IL-28A and IL-29. These three cytokines are clustered on chromosome 19q13. Their expression is triggered by viral infections. A heterodimeric class II cytokine receptor, composed of IL10RB and IL28RA, facilitates the interaction of all three cytokines.
Description
Recombinant human IL-28B, expressed in Sf9 insect cells, is a single glycosylated polypeptide chain. The protein sequence includes amino acids 22-196 of IL-28B, a C-terminal 6-His tag, totaling 181 amino acids with a molecular weight of 20.4 kDa. On SDS-PAGE under reducing conditions, IL-28B appears as multiple bands between 18-28 kDa. It is purified using proprietary chromatographic techniques.
Physical Appearance
A clear and sterile solution.
Formulation
The IL-28B protein is supplied at a concentration of 0.25 mg/ml in a solution containing phosphate-buffered saline (pH 7.4), 20% glycerol, 1 mM EDTA, and 0.1 mM PMSF.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store at -20°C. The addition of a carrier protein such as HSA or BSA (0.1%) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of IL-28B is greater than 85%, as assessed by SDS-PAGE analysis.
Synonyms
Lambda 3, IL28B, Interleukin 28B (IFN, Lambda 3), Cytokine Zcyto22, Interleukin-28B, Interleukin-28C, IFN-Lambda-3, IL-28B, IL-28C, IL28C, Lambda 4, Interleukin 28B, Interleukin 28C, IFN-Lambda-4, ZCYTO22, IFNL3.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
VPVARLRGAL PDARGCHIAQ FKSLSPQELQ AFKRAKDALE ESLLLKDCKC RSRLFPRTWD LRQLQVRERP VALEAELALT LKVLEATADT DPALGDVLDQ PLHTLHHILS QLRACIQPQP TAGPRTRGRL HHWLHRLQEA PKKESPGCLE ASVTFNLFRL LTRDLNCVAS GDLCVHHHHHH.
Product Science Overview
Introduction
Structure and Expression
Interleukin 28B is a secreted cytokine that belongs to the IL-28/IL-29 family . It is produced by a variety of cell types and shares many functions with Type I Interferons . The recombinant form of Interleukin 28B is typically expressed in Escherichia coli (E. coli) and is purified to high levels of purity .
Biological Activity
Mechanism of Action
Applications
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