IL 28A Mouse
IFN-lambda-2, Interleukin-28A, IL-28A, Ifnl2, Il28a, EG330496.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
The IL-28A is purified by proprietary chromatographic techniques.
Product Specs
Interleukin 28A (IL-28A), also known as interferon lambda 2 (IFN-lambda 2), is a cytokine that plays a role in the immune response to viral infections. IL-28A is part of the type III interferon family and shares similarities with type I interferons (IFNs) in its antiviral and antitumor activities. It is induced upon viral infection and signals through a receptor complex composed of IL10RB and IL28RA. IL-28A exhibits a more restricted range of activity compared to type I IFNs. Its antiviral effects are less potent than those of IFN-alpha or IFN-beta, and it acts on a smaller subset of cell types. IL-28A induces the production of chemokines like CXCL10, attracting immune cells to the site of infection. Additionally, it can promote the differentiation of dendritic cells (DCs) into a tolerogenic state, potentially modulating the immune response. Overall, IL-28A contributes to the innate immune response against viral infections, although its specific roles and mechanisms are still under investigation.
IFN-lambda-2, Interleukin-28A, IL-28A, Ifnl2, Il28a, EG330496.
Product Science Overview
IL-28A is a cytokine that exerts its biological effects through a receptor complex that also binds IL-28B and IL-29 . The recombinant form of IL-28A is typically expressed in Escherichia coli (E. coli) cells and purified using high-performance liquid chromatography (HPLC) and other stringent purification methods to ensure high purity and biological activity . The predicted molecular weight of recombinant mouse IL-28A is approximately 20.5 kDa, although it may appear as 25-30 kDa on SDS-PAGE due to post-translational modifications .
IL-28A has significant antiviral activities and stimulates antiviral responses through a heterodimeric receptor composed of IL-28 receptor alpha (IL-28RA) and IL-10 receptor beta (IL-10RB) . Upon binding to its receptor, IL-28A induces the phosphorylation of STAT1, STAT2, and STAT3, which are critical for the transcription of antiviral genes . The biological activity of recombinant mouse IL-28A is often assessed by its ability to activate STAT proteins and its antiviral effects in cell-based assays .
Recombinant mouse IL-28A is used extensively in research to study its role in antiviral immunity and its potential therapeutic applications in treating Th1-mediated inflammatory diseases, such as T-cell-mediated hepatitis . The lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After reconstitution, it can be stored at 2°C to 8°C for one month or at -20°C to -70°C for long-term storage . It is crucial to avoid repeated freeze-thaw cycles to maintain the protein’s stability and activity .
