IL 28A Human
Interleukin-28A, IL-28A, IFN-Lambda 2, IFN-Lambda 2, Cytokine ZCYTO20, IL28A, IFNL2, ZCYTO20.
(b) Analysis by SDS-PAGE.
Description
Product Specs
Interleukin 28A (IL-28A), a member of the type III interferon family, shares a distant relationship with type I interferons and the IL-10 family. Viral infections trigger the expression of IL-28A, which subsequently binds to a heterodimeric class II cytokine receptor composed of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha. IL-28A exhibits functional similarities to type I IFNs, including antiviral, antiproliferative, and in vivo antitumor activities. However, its potency is generally lower, and its activity is observed in a more limited range of cell lines. The genes encoding IFN-lambda 1, IFN-lambda 2, and IFN-lambda 3 are located in close proximity on human chromosome 19. IL-28A uniquely induces the mRNA expression of ELR(-) CXC chemokines in human peripheral blood mononuclear cells, independent of IFN-gamma. Furthermore, IL-28A possesses the ability to generate tolerogenic dendritic cells (DCs), a function that could potentially counteract the effects of IFN-beta. Produced in response to viral infection, IL-28A activates both monocytes and macrophages, leading to the production of a specific set of cytokines. This highlights its significant role in initiating innate immune responses at the site of viral infection.
Interleukin-28A, IL-28A, IFN-Lambda 2, IFN-Lambda 2, Cytokine ZCYTO20, IL28A, IFNL2, ZCYTO20.
Product Science Overview
IL-28A is a protein that is expressed in human cells and has a molecular weight of approximately 21 kDa . The recombinant form of IL-28A is typically produced in human 293 cells . The protein is lyophilized from a filtered solution of PBS, pH 7.2, and can be stored at -20°C for long-term stability .
IL-28A exhibits several important biological activities:
- Antiviral Activity: IL-28A has been shown to exhibit antiviral activities both in vitro and in vivo . It can induce an antiviral state by activating various antiviral proteins such as Mx proteins and 2’,5’-oligoadenylate synthetase .
- Anti-Tumor Activity: IL-28A directly inhibits tumor cell proliferation and promotes anti-tumor immune responses .
- Immune Modulation: IL-28A activates several signaling pathways, including Stat1, Stat3, and Stat5, which are crucial for immune responses .
IL-28A interacts with a heterodimeric receptor composed of the IL28-Rα and IL10-RI subunits . The expression of IL-28Rα is limited to a few cell types, including plasmacytoid dendritic cells and epithelial cells . This selective receptor distribution may result in fewer side effects compared to Type I Interferons .
Recombinant IL-28A is used in various research applications, including:
