IL 27 Human
Description
IL-27 Human Recombinant produced in HEK cells is a glycosylated non-covalently heterodimer that consists of 2 chains, IL27A (IL27p28) having a Mw of 25kDa and IL27B (EBI3) having a Mw of 30kDa. Each chain migrates separately on SDS-PAGE.
The IL-27 protein is purified by proprietary chromatographic techniques
Product Specs
Recombinant human IL-27 is a glycosylated heterodimer produced in HEK cells. It comprises two polypeptide chains: IL27A (IL27p28) with a molecular weight of 25 kDa and IL27B (EBI3) with a molecular weight of 30 kDa. These chains separate during SDS-PAGE analysis.
The purification of IL-27 protein is achieved through specialized chromatographic methods.
Avoid repeated freeze-thaw cycles.
FPRPPGRPQL SLQELRREFT VSLHLARKLL AEVRGQAHRF AESHLPGVNL YLLPLGEQLP DVSLTFQAWR RLSDPERLCF ISTTLQPFHA LLGGLGTQGR WTNMERMQLW AMRLDLRDLQ RHLRFQVLAA GFNLPEEEEE EEEEEEEERK GLLPGALGSA LQGPAQVSWP QLLSTYRLLH SLELVLSRAV RELLLLSKAG HSVWPLGFPT LSPQP.
IL-27B (209aa):
RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRPRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGK.
Product Science Overview
IL-27 plays a crucial role in the regulation of the immune response. It has both pro-inflammatory and anti-inflammatory properties, making it a versatile cytokine in immune modulation. Some of its key functions include:
- Promotion of T-cell differentiation: IL-27 induces the differentiation of naive CD4+ T cells into Th1 cells, which are essential for the immune response against intracellular pathogens .
- Inhibition of Th17 cell differentiation: IL-27 suppresses the differentiation of Th17 cells, which are involved in autoimmune diseases .
- Antiviral activity: IL-27 has been shown to induce a robust antiviral response, similar to interferons, by activating the JAK-STAT signaling pathway .
Recombinant human IL-27 is a synthetic form of the cytokine produced using recombinant DNA technology. It is optimized for use in various research applications, including cell culture, differentiation studies, and functional assays . The recombinant protein is typically produced in HEK293 cells and is available in research-grade quality, with high purity and low endotoxin levels .
Recombinant IL-27 is widely used in scientific research due to its diverse biological activities. Some of the key research areas include:
- Autoimmune diseases: Studying the role of IL-27 in the pathogenesis and potential treatment of autoimmune conditions .
- Cancer research: Investigating the ability of IL-27 to induce tumor-specific protective immunity and its potential as an immunotherapeutic agent .
- Viral infections: Exploring the antiviral properties of IL-27 and its potential use in combating viral infections .
