IL 23 Human, His

Interleukin-23 Human Recombinant, His Tag
Cat. No.
BT2921
Source

Sf9, Baculovirus cells.

Synonyms
Interleukin 23 alpha subunit p19, Interleukin-12 subunit beta p40, SGRF, IL23P19, IL-23-A, interleukin-six, G-CSF related factor, JKA3 induced upon T-cell activation, interleukin 12B (natural killer cell stimulatory factor 2 cytotoxic lymphocyte maturation factor 2 p40), NK cell stimulatory factor chain 2, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF2, CLMF p40.
Appearance
Sterile Filtered clear solution.
Purity

Greater than 95.0% as determined by analysis by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Recombinant Human Interleukin-23 produced in Sf9 Baculovirus cells is a glycosylated heterodimer composed of 2 disulfide-linked subunits. A p19 subunit which is unique to IL23 and a p40 subunit which is shared with IL12. The p19 subunit/IL23A (20-189aa, total of 176 aa, MW 19.5kDa) and p40 subunit /IL12B (23-328aa, total of 306 aa, MW 34.6kDa), the total predicted molecular mass of 54.1kDa (Molecular weight on SDS-PAGE will appear higher). IL23 is fused to a 6 aa His-Tag at C-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Interleukin-23 (IL-23) is a heterodimeric cytokine composed of two subunits: a unique p19 subunit (IL23A) and a p40 subunit (IL12B) shared with IL-12. Produced by antigen-presenting cells, IL-23 plays a crucial role in inflammatory responses and is implicated in autoimmune diseases. It binds to its receptor complex (IL-12Rβ1 and IL-23R) to activate STAT and NF-κB signaling pathways, leading to interferon-gamma production. Unlike IL-12, which primarily targets naive CD4(+) T cells, IL-23 preferentially acts on memory CD4(+) T cells.
Description
Recombinant Human Interleukin-23, expressed in Sf9 Baculovirus cells, is a glycosylated heterodimer composed of two disulfide-linked subunits: the p19 subunit (IL23A) and the p40 subunit (IL12B). The p19 subunit (20-189aa, 176 aa, 19.5 kDa) and the p40 subunit (23-328aa, 306 aa, 34.6 kDa) result in a predicted molecular weight of 54.1 kDa (appears higher on SDS-PAGE). A 6-aa His-tag is fused to the C-terminus of IL23. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The IL23 protein solution (1 mg/ml) is formulated in PBS (pH 7.4) and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), store at 4°C. For long-term storage, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 95.0% by SDS-PAGE analysis.
Synonyms
Interleukin 23 alpha subunit p19, Interleukin-12 subunit beta p40, SGRF, IL23P19, IL-23-A, interleukin-six, G-CSF related factor, JKA3 induced upon T-cell activation, interleukin 12B (natural killer cell stimulatory factor 2 cytotoxic lymphocyte maturation factor 2 p40), NK cell stimulatory factor chain 2, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF2, CLMF p40.
Source

Sf9, Baculovirus cells.

Amino Acid Sequence

IL12B: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS.

IL23A: RAVPGGSSPA WTQCQQLSQK LCTLAWSAHP LVGHMDLREE GDEETTNDVP HIQCGDGCDP QGLRDNSQFC LQRIHQGLIF YEKLLGSDIF TGEPSLLPDS PVGQLHASLL GLSQLLQPEG HHWETQQIPS LSPSQPWQRL LLRFKILRSL QAFVAVAARV FAHGAATLSP HHHHHH.

Product Science Overview

Structure and Function

IL-23 plays a crucial role in the immune system by activating the STAT and NF-kB pathways, which stimulate the production of interferon-gamma (IFN-γ) . This cytokine is involved in the proliferation and survival of T-helper 17 (Th17) cells, which are essential for the immune response against extracellular pathogens and are implicated in autoimmune diseases .

Recombinant IL-23

Recombinant IL-23 is produced using various expression systems, such as HEK293 cells . The recombinant form often includes a His tag, which is a sequence of histidine residues added to the protein to facilitate purification through affinity chromatography . This tag does not interfere with the protein’s biological activity and allows for high-purity preparations .

Applications

Recombinant IL-23 is widely used in research to study its role in immune responses and its potential as a therapeutic target for autoimmune diseases and cancer . It is tested in various applications, including Western Blot, Immunohistochemistry, ELISA, and Functional Assays .

Storage and Handling

Lyophilized recombinant IL-23 should be reconstituted in water to a protein concentration of 0.1-1.0 mg/mL and stored at -80°C to -20°C for extended periods . It is essential to minimize freeze-thaw cycles to maintain the protein’s stability and activity .

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Source
Sf9, Baculovirus cells.
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