IL23R Human

Interleukin-23 Receptor Human Recombinant
Cat. No.
BT12752
Source
Sf9, Baculovirus cells.
Synonyms
Interleukin 23 Receptor, IL-23 Receptor, IL-23R, IL23R.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Interleukin-23 Receptor Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 574 amino acids (24-355a.a.) and having a molecular mass of 65.3kDa (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).
IL23R is fused with a 239 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Interleukin-23 receptor (IL23R) plays a crucial role in the immune system's response to inflammation. It belongs to a family of cytokine receptors and works in conjunction with another receptor, IL12RB1, to enable signaling by the cytokine IL23A. Found on various immune cells like monocytes and T cells, IL23R is involved in activating downstream signaling pathways, particularly the JAK-STAT pathway. This activation is key for the proper functioning of specific immune cells, including T cells and NK cells. IL23R's role in the immune response makes it a potential target for understanding and treating autoimmune diseases and potentially cancer.
Description
Recombinant Human Interleukin-23 Receptor, produced in Sf9 insect cells, is a single polypeptide chain with a molecular weight of approximately 65.3 kDa. It consists of amino acids 24-355 of the IL23R protein sequence, fused with a 239 amino acid hIgG-His tag at the C-terminus. The protein is purified using proprietary chromatographic methods. Due to glycosylation, it may appear larger (70-100 kDa) on SDS-PAGE.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The IL23R protein is supplied in a solution containing 0.25mg/ml of protein in a buffer consisting of Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the IL23R protein solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To ensure stability during long-term storage, the addition of a carrier protein like HSA or BSA (0.1%) is advisable. Repeated cycles of freezing and thawing should be avoided to prevent protein degradation.
Purity
The purity of the IL23R protein is determined by SDS-PAGE analysis and is found to be greater than 90%.
Synonyms
Interleukin 23 Receptor, IL-23 Receptor, IL-23R, IL23R.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADPGITNINC SGHIWVEPAT IFKMGMNISI YCQAAIKNCQ PRKLHFYKNG IKERFQITRI NKTTARLWYK NFLEPHASMY CTAECPKHFQ ETLICGKDIS SGYPPDIPDE VTCVIYEYSG NMTCTWNAGK LTYIDTKYVV HVKSLETEEE QQYLTSSYIN ISTDSLQGGK KYLVWVQAAN ALGMEESKQL QIHLDDIVIP SAAVISRAET INATVPKTII YWDSQTTIEK VSCEMRYKAT TNQTWNVKEF DTNFTYVQQS EFYLEPNIKY VFQVRCQETG KRYWQPWSSL FFHKTPETVP QVTSKAFQHD TWNSGLTVAS ISTGHLTSDN RGDIGLEPKS CDKTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGKHH HHHH.

Product Science Overview

Introduction

Interleukin-23 receptor (IL-23R) is a type I cytokine receptor that plays a crucial role in the immune system. It is part of the type I cytokine receptor family, specifically the type 2 subfamily . IL-23R is essential for the signaling of Interleukin-23 (IL-23), a heterodimeric cytokine composed of the p40 subunit of IL-12 and a p19 subunit . IL-23 is primarily produced by antigen-presenting cells such as macrophages and dendritic cells .

Structure and Function

IL-23R pairs with the receptor molecule IL-12Rβ1 (IL12RB1), and both are required for IL-23 signaling . The receptor complex associates constitutively with Janus kinase 2 (JAK2) and binds to the transcription activator STAT3 in a ligand-dependent manner . This interaction is crucial for the downstream signaling pathways that lead to the activation and proliferation of T-cells, particularly the TH17 cell lineage .

Biological Significance

IL-23R is expressed by various immune cells, including monocytes, Th1, Th0, NK cells, and dendritic cells . The IL-23/IL-23R signaling axis is vital for the production and survival of TH17 cells, which are involved in the immune response against extracellular pathogens and play a role in autoimmune diseases . IL-23R’s interaction with IL-23 leads to the production of pro-inflammatory cytokines such as IL-17, which are essential for the immune response .

Recombinant IL-23R

Recombinant human IL-23R is produced using various expression systems, including HEK293 cells and Spodoptera frugiperda (Sf 21) cells . The recombinant protein is typically purified to a high degree of purity (≥95%) and is used in various research applications, including Western blotting, immunohistochemistry, and ELISA . The recombinant IL-23R is available in both carrier-free and carrier-containing formulations, depending on the specific research needs .

Applications and Research

Recombinant IL-23R is widely used in research to study the IL-23/IL-23R signaling pathway and its role in immune responses. It is also used to investigate the potential therapeutic targets for autoimmune diseases and inflammatory conditions. The recombinant protein’s ability to induce IL-17 secretion by mouse splenocytes is a key functional assay used to measure its activity .

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