IL 16 Human, (121 a.a.)

Recombinant human IL-16 (121 a.a.) is a non-glycosylated polypeptide chain consisting of 121 amino acids. The molecular weight of this protein is approximately 12.4 kDa . The amino acid sequence of IL-16 is as follows:

SAASASAASD VSVESTAEAT VCTVTLEKMS AGLGFSLEGG KGSLHGDKPL TINRIFKGAA SEQSETVQPG DEILQLGGTA MQGLTRFEAW NIIKALPDGP VTIVIRRKSL QSKETTAAGD S

IL-16 functions primarily as a chemoattractant for CD4+ T cells, monocytes, and eosinophils. It signals through the CD4 receptor, which is also the primary receptor for HIV . This cytokine undergoes proteolytic processing, resulting in two functional proteins. The secreted C-terminal peptide is responsible for the cytokine activity, while the N-terminal product may play a role in cell cycle control .

Recombinant human IL-16 (121 a.a.) is typically produced in Escherichia coli (E. coli) expression systems. The protein is purified using high-performance liquid chromatography (HPLC) and SDS-PAGE gel analyses to ensure a purity of ≥ 98% . The endotoxin concentration is maintained at <1 EU/µg to ensure its safety and efficacy in research applications .

IL-16 is widely used in research to study its role in immune responses and its potential therapeutic applications. It is particularly valuable in investigating T cell activation, chemoattraction, and HIV inhibition mechanisms .

Recombinant human IL-16 (121 a.a.) is typically lyophilized and should be stored at -20°C for long-term stability. Upon reconstitution, it is recommended to store the protein at 2-8°C for short-term use and at -20°C to -70°C for long-term storage . Avoid repeated freeze-thaw cycles to maintain its stability and activity .