IL 16 Mouse

Interleukin-16 Mouse Recombinant
Cat. No.
BT30926
Source
Escherichia Coli.
Synonyms
LCF, Lymphocyte Chemoattractant Factor, prIL-16, KIAA4048, mKIAA4048, Il16, IL-16, Interleukin-16.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 90.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Interleukin-16 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 127 amino acids and having a molecular mass of 13.2 kDa.
The Mouse IL-16 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Interleukin-16 (IL-16) is a cytokine with multiple functions. It attracts immune cells like T cells, monocytes, and eosinophils, influences T cell activation, and can suppress HIV replication. IL-16 signals through the CD4 receptor. This protein undergoes processing to produce two functional parts. The shorter, secreted part (C-terminal peptide) is responsible for the known functions of IL-16, while the longer part (N-terminal product) might be involved in regulating the cell cycle. The enzyme caspase 3 is believed to be involved in processing this protein. There are two slightly different versions of the IL-16 gene, resulting in two isoforms of the protein. In essence, IL-16 acts as a signal for specific immune cells, influencing their movement and behavior, and it also binds to CD4.
Description
This product is a recombinant version of mouse Interleukin-16, produced in E. coli bacteria. It is a single chain protein without any sugar molecules attached (non-glycosylated), consisting of 127 amino acids. The molecular weight of this protein is 13.2 kDa. The Mouse IL-16 is purified using specialized chromatographic techniques.
Physical Appearance
White powder that has been freeze-dried and sterilized by filtration.
Formulation
Before freeze-drying, the Murine IL-16 was in a solution containing 1 mg/ml of protein. This solution was extensively dialyzed against a buffer solution containing 10mM sodium phosphate at a pH of 7.5.
Solubility
To reconstitute the freeze-dried Murine IL16, it is recommended to dissolve it in sterile, highly purified water (18MΩ-cm H2O) at a concentration of at least 100 µg/ml. This solution can be further diluted with other aqueous solutions as needed.
Stability
While the freeze-dried Mouse IL-16 remains stable for 3 weeks at room temperature, it's best to store it in a dry environment below -18°C. Once reconstituted, it should be kept at 4°C and used within 2-7 days. For long-term storage, freeze it below -18°C, ideally with a carrier protein like HSA or BSA at a concentration of 0.1%. Avoid repeated freezing and thawing.
Purity
The purity of this product is over 90%, as determined by two methods: analysis by RP-HPLC and analysis by SDS-PAGE.
Synonyms
LCF, Lymphocyte Chemoattractant Factor, prIL-16, KIAA4048, mKIAA4048, Il16, IL-16, Interleukin-16.
Source
Escherichia Coli.
Amino Acid Sequence
MHDLNSSTDS AASASAASDI SVESKEATVC TVTLEKTSAG LGFSLEGGKG SLHGDKPLTI NRIFKGDRTG EMVQPGDEIL QLAGTAVQGL TRFEAWNVIK ALPDGPVTIV IRRTSLQCKQ TTASADS.

Product Science Overview

Structure and Synthesis

IL-16 is synthesized as a large, inactive precursor protein known as pro-IL-16. This precursor protein is approximately 80 kDa in size and is stored inside the cell until it is activated . The activation of pro-IL-16 involves proteolytic cleavage mediated by caspase-3, which results in the release of two functional proteins . The cytokine function of IL-16 is attributed to the secreted C-terminal region, which is about 14 kDa in size . The N-terminal product may play a role in cell cycle control .

Function

IL-16 functions as a chemoattractant, attracting immune cells to sites of inflammation . It also modulates T cell activation and acts as an inhibitor of HIV replication . The signaling process of IL-16 is mediated by the CD4 receptor . This cytokine is involved in various immune responses and has been associated with the development of multiple sclerosis, an inflammatory demyelinating disease of the central nervous system .

Recombinant IL-16

Recombinant IL-16 is produced using various expression systems, including HEK293 cells and E. coli . The recombinant protein is purified to ensure high purity and low endotoxin levels, making it suitable for research applications . Recombinant mouse IL-16 is a non-glycosylated protein containing 127 amino acids and has a molecular weight of approximately 13.2 kDa .

Applications

Recombinant IL-16 is used in various research applications, including:

  • Cellular assays
  • ELISA
  • Western blot
  • In vivo assays in mice

These applications help researchers study the role of IL-16 in immune responses and its potential therapeutic applications.

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