IL6ST Human
Sf9, Baculovirus cells.
Interleukin 6 signal transducer, oncostatin M receptor, IL6ST, CD130, CDw130, GP130, GP130-RAPS, IL6R-beta
Greater than 95% as determined by SDS-PAGE.
Description
IL6ST Human produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 605 amino acids (23-619 a.a) and having a molecular mass of 68.9kDa.
IL6ST is fused to an 8 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
The interleukin-6 signal transducer, also known as IL6ST, is a receptor belonging to the class 1 cytokine receptor family. IL6ST binds to IL-6, either through a membrane-bound or soluble IL-6R, initiating the formation of a complex. This complex brings together another IL6ST molecule and IL-6, resulting in the creation of a homodimer. Subsequently, signal transduction is triggered.
Human IL6ST, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain consisting of 605 amino acids (amino acids 23-619). It has a molecular weight of 68.9 kDa.
An 8 amino acid His tag is fused to the C-terminus of IL6ST. Purification is achieved using proprietary chromatographic techniques.
The IL6ST solution is supplied at a concentration of 0.25 mg/ml. It is formulated in a buffer containing 10% glycerol and Phosphate-Buffered Saline (pH 7.4).
To ensure optimal long-term storage, consider adding a carrier protein such as 0.1% HSA or BSA.
Avoid repeated freeze-thaw cycles to maintain product integrity.
The purity of the product is determined by SDS-PAGE analysis and is greater than 95%.
Interleukin 6 signal transducer, oncostatin M receptor, IL6ST, CD130, CDw130, GP130, GP130-RAPS, IL6R-beta
Sf9, Baculovirus cells.
ELLDPCGYIS PESPVVQLHS NFTAVCVLKE KCMDYFHVNA NYIVWKTNHF TIPKEQYTII NRTASSVTFT DIASLNIQLT CNILTFGQLE QNVYGITIIS GLPPEKPKNL SCIVNEGKKM RCEWDRGRET HLETNFTLKS EWATHKFADC KAKRDTPTSC TVDYSTVYFV NIEVWVEAEN ALGKVTSDHI NFDPVYKVKP NPPHNLSVIN SEELSSILKL TWTNPSIKSV IILKYNIQYR TKDASTWSQI PPEDTASTRS SFTVQDLKPF TEYVFRIRCM KEDGKGYWSD WSEEASGITY EDRPSKAPSF WYKIDPSHTQ GYRTVQLVWK TLPPFEANGK ILDYEVTLTR WKSHLQNYTV NATKLTVNLT NDRYVATLTV RNLVGKSDAA VLTIPACDFQ ATHPVMDLKA FPKDNMLWVE WTTPRESVKK YILEWCVLSD KAPCITDWQQ EDGTVHRTYL RGNLAESKCY LITVTPVYAD GPGSPESIKA YLKQAPPSKG PTVRTKKVGK NEAVLEWDQL PVDVQNGFIR NYTIFYRTII GNETAVNVDS SHTEYTLSSL TSDTLYMVRM AAYTDEGGKD GPEFTFTTPK FAQGEIELEH HHHHH
Product Science Overview
Interleukin-6 (IL-6) is a multifunctional cytokine that plays a crucial role in immune response, inflammation, and hematopoiesis. It is produced by various cell types, including T cells, macrophages, fibroblasts, and endothelial cells. The IL-6 signal transduction pathway is essential for mediating the biological effects of IL-6, which include the induction of acute phase responses, immune cell differentiation, and the regulation of metabolic, regenerative, and neural processes.
IL-6 exerts its effects through binding to its specific receptor, IL-6 receptor alpha (IL-6Rα), which exists in both membrane-bound and soluble forms. The binding of IL-6 to IL-6Rα leads to the formation of a complex with the signal transducing receptor subunit gp130. This receptor complex initiates intracellular signaling cascades, primarily through the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway .
The IL-6 signaling pathway can be divided into two main types: classic signaling and trans-signaling.
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Classic Signaling: This occurs when IL-6 binds to membrane-bound IL-6Rα, leading to the activation of gp130 and subsequent intracellular signaling. Classic signaling is typically associated with anti-inflammatory responses and is crucial for the regulation of immune homeostasis .
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Trans-Signaling: In this pathway, IL-6 binds to soluble IL-6Rα, forming a complex that can interact with gp130 on cells that do not express membrane-bound IL-6Rα. Trans-signaling is often linked to pro-inflammatory responses and is implicated in various chronic inflammatory diseases .
Recombinant human IL-6 is a laboratory-produced form of the cytokine, which is used in research and therapeutic applications. It is typically expressed in bacterial systems such as Escherichia coli and purified to high levels of purity. Recombinant IL-6 retains the biological activity of the native cytokine and is used to study its effects on various cell types and in different biological contexts .
Recombinant IL-6 has several applications in biomedical research and clinical practice:
- Research Tool: It is used to investigate the molecular mechanisms of IL-6 signaling, the role of IL-6 in disease pathogenesis, and the development of IL-6-targeted therapies.
- Therapeutic Agent: Recombinant IL-6 is being explored as a potential therapeutic agent for conditions such as cancer, autoimmune diseases, and chronic inflammatory disorders. It is also used in the development of vaccines and as an adjuvant to enhance immune responses .
