IL34 Mouse

Interleukin 34 Mouse Recombinant
Cat. No.
BT14427
Source
E.coli.
Synonyms
Interleukin-34, IL-34, Il34.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

IL34 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 236 amino acids (21-235 a.a) and having a molecular mass of 26.8kDa.
IL34 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Interleukin 34 (IL34) is a cytokine that plays a crucial role in the immune system by promoting the development and survival of monocytes and macrophages. These cells are important components of the innate immune response, which is the body's first line of defense against infection. IL34 exerts its effects by binding to the colony-stimulating factor-1 receptor (CSF-1R), which is found on the surface of monocytes and macrophages. By activating CSF-1R, IL34 triggers signaling pathways that regulate cell survival, proliferation, and differentiation. In addition to its role in monocyte and macrophage development, IL34 has been implicated in various other biological processes, including viral infection, adaptive immunity, and bone marrow cell proliferation. Its involvement in these diverse processes highlights the importance of IL34 in maintaining immune homeostasis and coordinating immune responses.
Description
Recombinant Mouse IL34 is a single, non-glycosylated polypeptide chain produced in E. coli. It consists of 236 amino acids, with a molecular weight of 26.8 kDa. The protein sequence spans from amino acid residues 21 to 235, excluding the signal peptide. To facilitate purification and detection, a 21 amino acid His-tag is fused to the N-terminus of the protein. The recombinant IL34 protein is purified using proprietary chromatographic techniques to ensure high purity.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The IL34 protein is supplied as a solution at a concentration of 0.5 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0) and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the protein solution can be stored at 4°C. For long-term storage, it is recommended to freeze the protein at -20°C. To enhance stability during long-term storage, adding a carrier protein like HSA or BSA to a final concentration of 0.1% is advisable. Avoid repeated freeze-thaw cycles to prevent protein degradation.
Purity
The purity of the IL34 protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
Interleukin-34, IL-34, Il34.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MNENLEIWTL TQDKECDLTG YLRGKLQYKN RLQYMKHYFP INYRIAVPYE GVLRVANITR LQKAHVSERE LRYLWVLVSL NATESVMDVL LEGHPSWKYL QEVQTLLENV QRSLMDVEIG PHVEAVLSLL STPGLSLKLV RPKALLDNCF RVMELLYCSC CKQSPILKWQ DCELPRLHPH SPGSLMQCTA TNVYPLSRQT PTSLPGSPSS SHGSLP.

Product Science Overview

Structure and Characteristics

Mouse IL-34 is synthesized as a 235 amino acid precursor, which includes a 20 amino acid signal sequence and a 215 amino acid mature chain . The mature protein has a predicted molecular mass of approximately 25.4 kDa, but it typically appears as a 35-40 kDa band under reducing conditions in SDS-PAGE due to glycosylation . The protein is noncovalently linked as a homodimer .

Biological Activity

IL-34 promotes the differentiation and viability of monocytes and macrophages through its interaction with CSF-1R. It shares structural homology with macrophage colony-stimulating factor (M-CSF) and induces myeloid cell expansion and differentiation in a manner similar to other colony-stimulating factors such as granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) . IL-34 is involved in the development of various myeloid compartments, including dendritic cells, monocytes, Langerhans cells, and microglia .

Expression and Regulation

IL-34 is expressed by several different tissues in response to inflammatory stimuli. It is particularly important in the bone marrow, where it plays a role in hematopoietic differentiation by inducing colony-forming units of macrophages and promoting osteoclast differentiation, adhesion, and proliferation .

Recombinant Production

Recombinant mouse IL-34 is produced in various expression systems, including yeast and mammalian cells. The recombinant protein is typically purified to a high degree of purity (>95%) and is tested for biological activity in appropriate bioassays . It is available in both carrier-free and carrier-containing formulations, with the latter often including bovine serum albumin (BSA) to enhance protein stability and shelf-life .

Applications

Recombinant mouse IL-34 is widely used in research to study its role in immune cell differentiation and function. It is utilized in cell culture, differentiation studies, and functional assays to investigate the signaling pathways and biological effects mediated by IL-34 and CSF-1R .

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IL34 Human, His
Interleukin 34 Human Recombinant, His Tag
Cat. No.
BT14253
Source
E.coli.
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