IL33 Antibody

Interleukin-33, Mouse Anti Human
Cat. No.
BT7979
Source
Synonyms
Interleukin 33, DVS27, NF-HEV, NKHEV, C9orf26, Interleukin-1 family member 11, IL- 1F11, Nuclear factor from high endothelial venules, NFEHEV, DKFZp586H0523, RP11-575C20.2, IL-33.
Appearance
Sterile Filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine that may also regulate gene transcription in producer cells. It is structurally similar to IL-1, inducing helper T cells to produce type 2 cytokines. IL-33 functions by binding to the IL1RL-1 receptor (also known as ST2), which activates NF-kappa-B and MAP kinases. This activation leads to the production of cytokines like IL-4, IL-5, and IL-13 by Th2 cells in vitro. In vivo, IL-33 can cause significant pathological changes in mucosal organs. Notably, caspase-1 can cleave IL-33 into a 12kDa N-terminal fragment and an 18kDa C-terminal fragment.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
Supplied as a 1 mg/ml solution in PBS, pH 7.4, containing 0.1% sodium azide.
Storage Procedures
Store at 4°C for up to 1 month. For long-term storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
Stable for 12 months at -20°C. Stable for 1 month at 4°C.
Applications
This IL33 antibody has been validated for ELISA and Western blot applications, demonstrating high specificity and reactivity. However, optimal working dilutions should be determined empirically for each application. For Western blot analysis, a dilution range of 1:500 to 1:1000 is recommended, with 1:500 as a starting point.
Synonyms
Interleukin 33, DVS27, NF-HEV, NKHEV, C9orf26, Interleukin-1 family member 11, IL- 1F11, Nuclear factor from high endothelial venules, NFEHEV, DKFZp586H0523, RP11-575C20.2, IL-33.
Purification Method
IL33 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
P4E9AT.
Immunogen
Anti-human IL33 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with Recombinant human IL33 amino acids 112-270 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.

Product Science Overview

Introduction to Interleukin-33 (IL-33)

Interleukin-33 (IL-33) is a cytokine belonging to the IL-1 family, which plays a crucial role in the immune system by acting as an alarm signal (alarmin) released upon cellular damage or stress. It is encoded by the IL-33 gene located on chromosome 9 in humans and chromosome 19 in mice . IL-33 is primarily expressed in the nuclei of endothelial and epithelial cells and is involved in various biological processes, including inflammation, immune response, and tissue homeostasis .

Biological Functions of IL-33

IL-33 exerts its effects by binding to the ST2 receptor (suppression of tumorigenicity 2), which is expressed on various immune cells such as T-helper 2 (Th2) cells, mast cells, eosinophils, and innate lymphoid cells . The IL-33/ST2 axis is pivotal in mediating immune responses, particularly in allergic and inflammatory diseases. IL-33 has been implicated in the pathogenesis of conditions such as asthma, chronic obstructive pulmonary disease (COPD), and age-related macular degeneration (AMD) .

Mouse Anti-Human IL-33 Antibodies

Mouse anti-human IL-33 antibodies are monoclonal antibodies developed to specifically target and neutralize human IL-33. These antibodies are produced in hybridomas and purified through affinity chromatography . They are widely used in research and clinical settings to study the role of IL-33 in various diseases and to develop potential therapeutic interventions.

Applications in Research and Medicine
  1. Biomarker Assays: IL-33 is a valuable biomarker for several inflammatory diseases. Sensitive and specific assays, such as enzyme-linked immunosorbent assays (ELISA) and immuno-polymerase chain reaction (iPCR) assays, have been developed to detect IL-33 levels in various human matrices .
  2. Therapeutic Target: Given its role in inflammatory diseases, IL-33 is considered a potential target for biological therapies. Neutralizing antibodies against IL-33 are being explored for their therapeutic potential in conditions like COPD and asthma .
  3. Neurological Research: Recent studies have shown that exogenous IL-33 can enhance lymphatic drainage in the brain and improve motor and memory skills in traumatic brain injury (TBI) models .

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IL 33 Human
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Source
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