IL31 Mouse
Interleukin-31 Mouse Recombinant
Cat. No.
BT13965
Source
Escherichia Coli.
Synonyms
Interleukin 31, IL31, IL-31.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
IL31 mouse recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 141 amino acids and having a molecular mass of 15.7 kDa.
Product Specs
Introduction
IL-31, primarily produced by activated Th2 cells, interacts with a receptor complex comprising IL-31RA and Oncostatin M Receptor (OSMR), which is constitutively expressed on epithelial cells and keratinocytes. This cytokine plays a crucial role in allergic skin disorders and the modulation of other allergic diseases, such as asthma. IL-31 is notably involved in the sensation of itch and promotes scratching behavior in NC/Nga mice, a model for atopic dermatitis. Its expression is linked to CLA(+) T cells and contributes to skin inflammation and pruritus associated with atopic dermatitis. Moreover, IL-31 acts as a potent inducer of pro-inflammatory mediators in human colonic subepithelial myofibroblasts (SEMFs) and functions as a pro-inflammatory cytokine derived from Th2 cells. Elevated serum IL-31 levels are observed in patients with atopic dermatitis. Notably, IL-31 interacts with a wide range of immune and non-immune cells, suggesting pleiotropic physiological functions, including the regulation of hematopoiesis and immune responses.
Description
Recombinant mouse IL-31, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 141 amino acids. It has a molecular weight of 15.7 kDa.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The IL31 protein solution (1mg/ml) was lyophilized from a 10mM sodium phosphate buffer at pH 7.5.
Solubility
To reconstitute the lyophilized IL31, it is recommended to dissolve it in sterile 18 megaohm-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted into other aqueous solutions as needed.
Stability
Lyophilized IL31 Recombinant protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -18°C or lower. After reconstitution, the IL31 solution should be stored at 4°C for short-term use (2-7 days) and at -18°C or lower for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of this protein is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
Interleukin 31, IL31, IL-31.
Source
Escherichia Coli.
Amino Acid Sequence
MTCSLSFGAP ISKEDLRTTI DLLKQESQDL YNNYSIKQAS GMSADESIQL PCFSLDREAL TNISVIIAHL EKVKVLSENT VDTSWVIRWL TNISCFNPLN LNISVPGNTD ESYDCKVFVL TVLKQFSNCM AELQAKDNTT C.
Product Science Overview
Structure and Expression
Receptor and Signaling Pathways
IL-31 exerts its biological effects through a heterodimeric receptor complex composed of IL-31 receptor A (IL-31RA) and oncostatin M receptor (OSMR) . Upon binding to its receptor, IL-31 activates several intracellular signaling pathways, including the JAK/STAT, PI3K/AKT, and MAPK pathways . These pathways are crucial for mediating the cytokine’s effects on cell proliferation, differentiation, and survival.
Biological Functions
IL-31 is involved in various biological processes, including:
- Immune Regulation: IL-31 plays a role in modulating immune responses, particularly in the context of allergic diseases such as atopic dermatitis and asthma .
- Skin Inflammation: Overexpression of IL-31 in transgenic mice leads to pruritus (itching), alopecia (hair loss), and skin lesions, indicating its role in skin inflammation and barrier dysfunction .
- Hematopoiesis: IL-31 has been shown to enhance the survival of myeloid progenitor cells, suggesting a role in hematopoiesis .
Research and Therapeutic Potential
Given its involvement in inflammatory and allergic responses, IL-31 is a potential target for therapeutic interventions aimed at treating conditions such as atopic dermatitis and other pruritic (itchy) skin disorders . Recombinant IL-31 proteins, such as those expressed in Escherichia coli, are used in research to study the cytokine’s functions and to develop potential therapeutic agents .
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