IL31 Canine, His
Sf9, Baculovirus cells.
IL-31, Interleukin 31, IL31.
Sterile filtered colorless solution.
Greater than 95.0% as determined by SDS-PAGE.
Description
IL31 Canine Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 142 amino acids (24-159a.a.) and having a molecular mass of 16.1kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa).
IL31 is fused to a 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
IL-31, a cytokine primarily produced by activated Th2 cells, interacts with a receptor complex comprising IL-31 Receptor Anatagonist and Onconstatin-M Receptor. This receptor complex is constitutively expressed on epithelial cells and keratinocytes. Functionally, IL-31 is implicated in the pathogenesis of allergic skin disorders and plays a role in regulating other allergic diseases such as asthma. Studies in NC/Nga mice, an animal model of atopic dermatitis, suggest that IL-31 is involved in itching sensation and promotes scratching behavior. IL-31 expression is linked to CLA(+) T cells and is believed to contribute to skin inflammation and pruritus associated with atopic dermatitis. Additionally, IL-31 acts as a potent inducer of proinflammatory mediators in human colonic subepithelial myofibroblasts (SEMFs), highlighting its proinflammatory role. Elevated serum IL-31 levels have been observed in patients with atopic dermatitis. Beyond its role in allergic responses, IL-31 exhibits pleiotropic physiological functions, influencing diverse immune and non-immune cells. These functions include regulation of hematopoiesis and immune response, involvement in inflammatory bowel disease, and contributions to airway hypersensitivity and dermatitis.
Recombinant Canine IL31, expressed in Sf9 Baculovirus cells, is a single glycosylated polypeptide chain consisting of 142 amino acids (residues 24-159). It has a molecular mass of 16.1kDa, although it may appear between 18-28kDa on SDS-PAGE due to glycosylation. The protein includes a 6 amino acid His-Tag fused at the C-terminus to facilitate purification, which is achieved through proprietary chromatographic methods.
A clear, colorless solution that has been sterilized by filtration.
The IL31 Canine protein solution is provided at a concentration of 1mg/ml in a buffer consisting of phosphate buffered saline (pH 7.4) and 10% glycerol.
The purity of the IL31 Canine protein is determined to be greater than 95% using SDS-PAGE analysis.
IL-31, Interleukin 31, IL31.
Sf9, Baculovirus cells.
SHMAPTHQLP PSDVRKIILE LQPLSRGLLE DYQKKETGVP ESNRTLLLCL TSDSQPPRLN SSAILPYFRA IRPLSDKNII DKIIEQLDKL KFQHEPETEI SVPADTFECK SFILTILQQF SACLESVFKS LNSGPQHHHH HH.
Product Science Overview
Interleukin-31 (IL-31) is a cytokine that belongs to the gp130/interleukin-6 cytokine family. It is primarily produced by activated T helper 2 (Th2) cells and cutaneous lymphocyte antigen-positive skin-homing T cells. IL-31 plays a significant role in immune response regulation and is involved in various allergic and inflammatory conditions.
Recombinant canine IL-31 is a protein that has been engineered to include a His-tag at its C-terminus. This His-tag facilitates the purification and detection of the protein. The recombinant protein corresponds to the amino acids 24-159 of the canine IL-31 sequence . It is typically expressed in baculovirus-infected insect cells, which allows for proper folding and post-translational modifications.
IL-31 signals through a heterodimeric receptor complex composed of IL-31 receptor A (IL-31RA) and the oncostatin M receptor (OSMR). This receptor complex is constitutively expressed on epithelial cells and keratinocytes and can be upregulated by interferon-gamma (IFN-γ) on monocytes . The interaction of IL-31 with its receptor leads to the activation of various downstream signaling pathways, including the JAK/STAT, PI3K/AKT, and MAPK pathways.
IL-31 is implicated in the promotion of allergic skin disorders, such as atopic dermatitis, and other allergic diseases, including asthma . In dogs, IL-31 has been associated with pruritus (itchiness) and other dermatological conditions. The recombinant form of canine IL-31 is used in research to study these conditions and to develop potential therapeutic interventions.
The recombinant canine IL-31 protein is produced using baculovirus expression systems in insect cells. This method ensures high yield and proper protein folding. The His-tag at the C-terminus of the protein allows for easy purification using nickel affinity chromatography. The purity of the recombinant protein is typically greater than 95%, as determined by SDS-PAGE .
Recombinant canine IL-31 is used in various research applications, including:
- Studying the role of IL-31 in allergic and inflammatory diseases: Researchers use the recombinant protein to investigate the mechanisms by which IL-31 contributes to conditions like atopic dermatitis and asthma.
- Developing therapeutic interventions: The protein is used to screen potential drugs and therapeutic agents that can modulate IL-31 activity.
- Understanding receptor interactions: Studies involving recombinant IL-31 help elucidate the interactions between IL-31 and its receptor complex, providing insights into the signaling pathways involved.
