IL2 Feline
Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2.
Greater than 95% as determined by SDS-PAGE.
Description
IL2 Feline Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 157 amino acids (21-154 a.a) and having a molecular mass of 17.8kDa. IL2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Interleukin 2 (IL2) is a cytokine crucial for the growth and proliferation of T and B lymphocytes, which are essential components of the immune system. The IL2 receptor, a protein complex on the surface of these cells, binds to IL2, triggering their activation and expansion. This cytokine's significance is underscored by its role in immune responses to antigens and the prevention of inflammatory bowel diseases.
Recombinant Feline IL2, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 157 amino acids, with a molecular weight of 17.8 kDa. This IL2 protein is modified with a 23 amino acid His-tag at the N-terminus to facilitate purification via specialized chromatographic techniques.
The IL2 protein solution has a concentration of 0.25 mg/ml. It is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 0.15M NaCl, and 30% glycerol.
For short-term storage (2-4 weeks), keep refrigerated at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein such as 0.1% HSA or BSA is recommended for long-term storage to enhance stability. Minimize repeated freezing and thawing to preserve protein integrity.
SDS-PAGE analysis confirms a purity level exceeding 95%.
The biological activity of this IL2 protein is determined by its ability to stimulate the proliferation of CTLL2 mouse cytotoxic T cells. In this assay, the ED50 value, representing the concentration at which half-maximal proliferation is observed, is less than or equal to 0.3 ng/ml.
Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2.
MGSSHHHHHH SSGLVPRGSH MGSAPASSST KETQQQLEQL LLDLRLLLNG VNNPENPKLS RMLTFKFYVP KKATELTHLQ CLVEELKPLE EVLYLAQSKN FHLNHIKELM SNINVTVLKL KGSETRFTCN YDDETATIVE FLNKWITFCQ SIFSTLT.
Product Science Overview
Interleukin-2 Feline Recombinant is typically produced in Escherichia coli (E. coli), a common bacterium used in biotechnology for protein production. The recombinant IL-2 is a single, non-glycosylated polypeptide chain containing 157 amino acids and has a molecular mass of approximately 17.8 kDa . It is often fused to a His-tag at the N-terminus to facilitate purification through chromatographic techniques .
IL-2 functions as an immunomodulating agent, meaning it modulates the activity of the immune system. Specifically, IL-2 stimulates the growth and activity of T-cells, which are a type of white blood cell that plays a critical role in the immune response. By enhancing the activity of T-cells, IL-2 can help the immune system to target and destroy cancer cells.
One of the primary veterinary applications of Interleukin-2 Feline Recombinant is in the treatment of fibrosarcoma in cats. Fibrosarcoma is an aggressive type of tumor that affects the soft tissues. The recombinant IL-2 is used in combination with surgery and radiotherapy to reduce the risk of tumor recurrence and to delay the progression of the disease .
The treatment regimen typically involves administering IL-2 around the site of the surgically removed tumor. The IL-2 is delivered using a carrier canarypox virus that contains the gene for feline IL-2. This virus does not replicate in cats but allows for the localized production of IL-2 at the injection site, thereby stimulating the immune response against any remaining cancer cells .
Clinical studies have demonstrated that the use of Interleukin-2 Feline Recombinant can significantly improve the outcomes for cats with fibrosarcoma. The treatment has been shown to reduce the risk of tumor relapse and increase the time to relapse when used in conjunction with surgery and radiotherapy .
As with any medical treatment, there are potential risks and side effects associated with the use of Interleukin-2 Feline Recombinant. Common side effects may include mild to moderate inflammation at the injection site. It is important for veterinarians to monitor the treated animals closely and manage any adverse reactions appropriately .
