IL18RAP Human
Sf9, Insect cells.
Interleukin-18 receptor accessory protein, IL-18 receptor accessory protein,
IL-18RAcP, Accessory protein-like, AcPL, CD218 antigen-like family member B, CDw218b, IL-1R accessory protein-like, IL-1RAcPL, Interleukin-1 receptor 7, IL-1R-7, IL-1R7, Interleukin-18 receptor accessory protein-like, Interleukin-18 receptor beta, IL-18R-beta, IL-18Rbeta, CD218b, IL1R7.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
IL18RAP produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 576 amino acids (20-356 a.a.) and having a molecular mass of 65.4kDa.
IL18RAP is expressed with an 239 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Interleukin-18 receptor accessory protein, IL-18 receptor accessory protein,
IL-18RAcP, Accessory protein-like, AcPL, CD218 antigen-like family member B, CDw218b, IL-1R accessory protein-like, IL-1RAcPL, Interleukin-1 receptor 7, IL-1R-7, IL-1R7, Interleukin-18 receptor accessory protein-like, Interleukin-18 receptor beta, IL-18R-beta, IL-18Rbeta, CD218b, IL1R7.
Sf9, Insect cells.
FNISGCSTKK LLWTYSTRSE EEFVLFCDLP EPQKSHFCHR NRLSPKQVPE HLPFMGSNDL SDVQWYQQPS NGDPLEDIRK SYPHIIQDKC TLHFLTPGVN NSGSYICRPK MIKSPYDVAC CVKMILEVKP QTNASCEYSA SHKQDLLLGS TGSISCPSLS CQSDAQSPAV TWYKNGKLLS VERSNRIVVD EVYDYHQGTY VCDYTQSDTV SSWTVRAVVQ VRTIVGDTKL KPDILDPVED TLEVELGKPL TISCKARFGF ERVFNPVIKW YIKDSDLEWE VSVPEAKSIK STLKDEIIER NIILEKVTQR DLRRKFVCFV QNSIGNTTQS VQLKEKRLEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH.
Product Science Overview
Interleukin 18 Receptor Accessory Protein (IL18RAP) is a crucial component of the interleukin-18 (IL-18) receptor complex. IL-18 is a pro-inflammatory cytokine that plays a significant role in the immune response by inducing the production of interferon-gamma (IFN-γ) and promoting T-helper 1 (Th1) cell responses . The IL-18 receptor complex consists of two subunits: IL-18 receptor alpha (IL18R1) and IL18RAP .
IL18RAP is an accessory subunit that enhances the binding affinity of IL-18 to its receptor, thereby facilitating signal transduction . This protein does not directly bind IL-18 but is essential for the high-affinity binding and subsequent activation of downstream signaling pathways . The binding of IL-18 to its receptor complex leads to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and activator protein 1 (AP-1), which are critical transcription factors involved in the inflammatory response .
Recombinant human IL18RAP is produced using recombinant DNA technology, typically in Escherichia coli (E. coli) expression systems . The recombinant protein is purified to high levels of purity and is used in various research applications to study the IL-18 signaling pathway and its role in immune responses .
IL18RAP plays a vital role in mediating the effects of IL-18 in various immune cells, including macrophages, neutrophils, and T cells . It has been implicated in several inflammatory and autoimmune diseases, such as systemic lupus erythematosus (SLE), where elevated levels of IL18RAP have been observed in neutrophils . The upregulation of IL18RAP in these cells is associated with increased production of reactive oxygen species (ROS) and enhanced disease activity .
Understanding the role of IL18RAP in the IL-18 signaling pathway has significant clinical implications. Targeting IL18RAP or its interaction with IL-18 could provide therapeutic strategies for treating inflammatory and autoimmune diseases . Additionally, recombinant IL18RAP can be used as a tool in research to further elucidate the mechanisms of IL-18-mediated immune responses .
