IL-10 Human, Sf9
Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10, GVHDS, IL10A, TGIF, T-Cell Growth Inhibitory Factor.
Greater than 95.0% as determined by SDS-PAGE.
Description
IL-10 Human produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 166 amino acids (19-178 aa) and having a molecular mass of 19.4kDa. IL-10 is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Interleukin 10, also known as IL-10 or human cytokine synthesis inhibitory factor, is a cytokine with anti-inflammatory properties. The IL10 gene in humans encodes for IL-10. Its receptor complex consists of two IL-10 receptor-1 and two IL-10 receptor-2 proteins, totaling four IL-10 receptor molecules. Upon binding to the receptor, IL-10 initiates STAT3 signaling by phosphorylating the cytoplasmic ends of IL-10 receptor 1 and IL-10 receptor 2 via JAK1 and Tyk2.
Produced in Sf9 Baculovirus cells, IL-10 Human is a single, glycosylated polypeptide chain with a molecular weight of 19.4kDa. It comprises 166 amino acids (19-178 aa). The protein includes a 6 amino acid His tag at the C-terminus and undergoes purification using proprietary chromatographic techniques.
The IL-10 solution is provided at a concentration of 0.5mg/ml in a buffer containing 10% Glycerol and Phosphate-Buffered Saline (pH 7.4).
For short-term storage (2-4 weeks), keep the vial refrigerated at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. To maintain product integrity, avoid repeated cycles of freezing and thawing.
SDS-PAGE analysis confirms a purity greater than 95.0%.
Biological activity is determined through a cell proliferation assay using MC/9 mouse mast cells. The ED50 is observed to be less than 5 ng/ml.
Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10, GVHDS, IL10A, TGIF, T-Cell Growth Inhibitory Factor.
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Product Science Overview
Human Recombinant IL-10 is produced in Sf9 Baculovirus cells. The recombinant protein is a single, glycosylated polypeptide chain containing 166 amino acids, with a molecular mass of approximately 19.4 kDa. It is fused to a 6 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques .
The biological activity of IL-10 is measured in a cell proliferation assay using MC/9 mouse mast cells. The effective dose (ED50) range is less than 5 ng/ml . IL-10 binds to its receptor complex, which consists of two IL-10 receptor-1 and two IL-10 receptor-2 proteins. This binding activates the STAT3 signaling pathway through the phosphorylation of the cytoplasmic ends of IL-10 receptor 1 and IL-10 receptor 2 via JAK1 and Tyk2 .
The IL-10 solution is formulated with 10% glycerol and phosphate-buffered saline (pH 7.4). For short-term storage, it is recommended to store the solution at 4°C if it will be used within 2-4 weeks. For long-term storage, it should be frozen at -20°C, and it is advisable to add a carrier protein (0.1% HSA or BSA) to prevent multiple freeze-thaw cycles .
