IL-20 is synthesized as a 176 amino acid precursor, which includes a 24 amino acid signal sequence and a 152 amino acid mature segment . The protein is mainly produced by myeloid cells such as monocytes, granulocytes, and dendritic cells, but it can also be produced by keratinocytes and fibroblasts . The expression of IL-20 is stimulated by various cytokines, including IL-1β, IL-17, IL-22, TNF, and LPS .
IL-20 requires an IL-β-subunit receptor (IL-20RB) for signaling, which can form a functional heterodimeric receptor with either the α-subunit of the IL-20 receptor (IL-20RA) or the α1-subunit of the IL-22 receptor (IL-22RA1) . These receptor variants allow efficient IL-20 signaling. Receptors for IL-20 are expressed in various tissues, including the skin, lungs, ovary, testes, and placenta .
IL-20 is involved in several biological processes, including:
Recombinant human IL-20 is produced using E. coli expression systems. The recombinant protein is typically purified to a high degree of purity (>97%) and is used in various research applications . It is available in both carrier-free and carrier-containing formulations, with Bovine Serum Albumin (BSA) often used as a carrier protein to enhance stability and shelf-life .
Recombinant IL-20 is used in cell proliferation assays and other research studies to understand its role in immune responses and potential therapeutic applications .