IL10RA Human, Active
Interleukin 10 Receptor, Alpha, IL10R, Interleukin-10 Receptor Subunit 1, IL-10 Receptor Subunit Alpha, IL-10R Subunit Alpha, IL-10R Subunit 1, CDW210A, IL-10R1, IL-10RA, Interleukin-10 Receptor Subunit Alpha, Interleukin-10 Receptor Alpha Chain, CD210 Antigen, HIL-10R, CD210a, CD210, IBD28, IL10RA.
Greater than 90.0% as determined by SDS-PAGE.
Description
IL10RA Human produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 220 amino acids (22-235 aa) and having a molecular mass of 25.2kDa.
IL10RA is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Interleukin 10 receptor, alpha subunit, also known as CDW210A, is a component of the IL-10 receptor and is encoded by the IL10RA gene in humans. As a receptor for interleukin 10 (IL-10), IL10RA belongs to the interferon receptor family. It plays a crucial role in suppressing the synthesis of interferon receptors by participating in the immunosuppressive signaling pathway of IL-10. Furthermore, IL10RA is involved in the insulin receptor substrate-2/PI 3-kinase/AKT pathway and the phosphorylation of JAK1 and TYK2 kinases.
IL10RA Human, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain composed of 220 amino acids (22-235 aa). With a molecular weight of 25.2 kDa, it features a 6 amino acid His tag fused at the C-terminus. The protein is purified using proprietary chromatographic techniques.
The IL10RA solution is provided at a concentration of 0.2 mg/ml in a buffer consisting of 20% Glycerol and Phosphate-Buffered Saline (pH 7.4).
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. To maintain product integrity, avoid repeated freeze-thaw cycles.
The purity of IL10RA Human is determined to be greater than 90.0% using SDS-PAGE analysis.
The biological activity of IL10RA Human is assessed based on its ability to inhibit proliferation in MC/9 mouse mast cells. The ED50 for this inhibitory effect, in the presence of Human IL-10, is determined to be less than or equal to 300 ng/ml.
Interleukin 10 Receptor, Alpha, IL10R, Interleukin-10 Receptor Subunit 1, IL-10 Receptor Subunit Alpha, IL-10R Subunit Alpha, IL-10R Subunit 1, CDW210A, IL-10R1, IL-10RA, Interleukin-10 Receptor Subunit Alpha, Interleukin-10 Receptor Alpha Chain, CD210 Antigen, HIL-10R, CD210a, CD210, IBD28, IL10RA.
HGTELPSPPS VWFEAEFFHH ILHWTPIPNQ SESTCYEVAL LRYGIESWNS ISNCSQTLSY DLTAVTLDLY HSNGYRARVR AVDGSRHSNW TVTNTRFSVD EVTLTVGSVN LEIHNGFILG KIQLPRPKMA PANDTYESIF SHFREYEIAI RKVPGNFTFT HKKVKHENFS CVQVKPSVAS RSNKGMWSKE ECISLTRQYF TVTNHHHHHH
Product Science Overview
Interleukin 10 Receptor Alpha (IL-10Rα) is a critical component of the immune system, playing a pivotal role in mediating the anti-inflammatory effects of Interleukin 10 (IL-10). This receptor is part of the type II cytokine receptor family and is essential for maintaining immune homeostasis and regulating inflammatory responses.
IL-10Rα is a transmembrane glycoprotein that forms a heterotetrameric complex with IL-10Rβ upon binding to its ligand, IL-10 . The receptor consists of two alpha (α) and two beta (β) subunits. The alpha subunit is primarily expressed in hematopoietic cells such as B cells, T cells, natural killer (NK) cells, monocytes, and macrophages . It is not typically found in non-hematopoietic cells like fibroblasts or endothelial cells .
When IL-10 binds to IL-10Rα, it induces a conformational change in the receptor, allowing IL-10Rβ to also bind IL-10, forming a stable heterotetrameric complex . This complex activates the Janus kinase 1 (JAK1) and Tyrosine kinase 2 (TYK2) signaling pathways . JAK1 associates with the alpha subunit, while TYK2 binds to the beta subunit, leading to the phosphorylation of specific tyrosine residues in the intracellular domain of IL-10Rα . This phosphorylation event triggers the activation of the Signal Transducer and Activator of Transcription 3 (STAT3), which dimerizes and translocates to the nucleus to induce the transcription of target genes .
IL-10Rα is crucial for suppressing inflammatory responses and modulating immune reactions mediated by T helper 1 (Th1) cells . It also plays a role in regulating the functional responses of neutrophils . Additionally, IL-10Rα-mediated activation of STAT3 inhibits starvation-induced autophagy, highlighting its role in cellular metabolism and survival .
Mutations or deficiencies in IL-10Rα can lead to severe immune dysregulation and are associated with various inflammatory and autoimmune diseases . For instance, defects in IL-10Rα have been linked to inflammatory bowel disease (IBD) and other chronic inflammatory conditions . Recombinant human IL-10Rα is used in research to study these pathways and develop potential therapeutic interventions.
Recombinant human IL-10Rα is produced using various expression systems, including HEK293 cells . These recombinant proteins are used in laboratory research to investigate the receptor’s function, signaling mechanisms, and potential therapeutic applications. The recombinant form retains the biological activity of the native protein and is used to study its role in immune regulation and inflammation .
