IgG Mouse, His
Description
IgG is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
KNTEPVLDSD GSYFMYSKLR VEKKNWVERN SYSCSVVHEG LHNHHTTKSF SRTPGKHHHH HH.
Product Science Overview
The Immunoglobulin Heavy Chain Constant Region Gamma 2a (Mouse Recombinant, His Tag) is a recombinant protein derived from the mouse immunoglobulin heavy chain. This protein is significant in various research and clinical applications due to its role in the immune response.
The Immunoglobulin Heavy Chain Constant Region Gamma 2a is a part of the immunoglobulin G (IgG) class of antibodies. It is composed of a single polypeptide chain containing 242 amino acids, with a molecular mass of approximately 27.5 kDa . The protein is fused to a 6-amino acid His-tag at the C-terminus, which facilitates its purification through affinity chromatography techniques .
The Immunoglobulin Heavy Chain Constant Region Gamma 2a plays a critical role in the immune system. It is involved in the effector functions of antibodies, such as complement activation and binding to Fc receptors on immune cells . These functions are essential for the clearance of pathogens and the regulation of immune responses.
In research, this recombinant protein is used in various assays and experiments to study antibody functions, interactions with other immune components, and the mechanisms of immune responses. It is also utilized in the development of therapeutic antibodies and diagnostic tools.
The recombinant protein is provided as a sterile filtered colorless solution, typically at a concentration of 1 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0) and 10% glycerol . For short-term storage, it can be kept at 4°C if used within 2-4 weeks. For long-term storage, it is recommended to store the protein at -20°C, with the addition of a carrier protein such as 0.1% HSA or BSA to prevent degradation . It is important to avoid multiple freeze-thaw cycles to maintain the protein’s stability and functionality.
