IgG Mouse
Description
IgG is purified by proprietary chromatographic techniques.
Product Specs
KNTEPVLDSD GSYFMYSKLR VEKKNWVERN SYSCSVVHEG LHNHHTTKSF SRTPGK.
Product Science Overview
Immunoglobulins, also known as antibodies, are glycoproteins produced by B-lymphocytes and plasma cells. They play a crucial role in the immune response by identifying and neutralizing pathogens such as bacteria and viruses. The immunoglobulin molecule consists of two heavy chains and two light chains, forming a Y-shaped structure. The heavy chains are classified into different classes based on their constant regions, which determine the antibody’s isotype and function.
The constant region of the immunoglobulin heavy chain is responsible for mediating various effector functions, such as binding to cell surface receptors and complement proteins. In mice, the immunoglobulin heavy chain constant region gamma 2a (IgG2a) is one of the subclasses of the IgG isotype. This subclass is known for its ability to mediate antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC), making it an important component of the immune response.
Recombinant immunoglobulins are produced using genetic engineering techniques, allowing for the expression of specific antibody fragments or entire antibodies in various host systems. The recombinant immunoglobulin heavy chain constant region gamma 2a (mouse) is a genetically engineered protein that mimics the natural IgG2a constant region found in mice. This recombinant protein is used in various research applications, including the study of antibody structure and function, the development of therapeutic antibodies, and the investigation of immune responses in mouse models.
The production of recombinant immunoglobulin heavy chain constant region gamma 2a involves the insertion of the gene encoding the IgG2a constant region into an expression vector. This vector is then introduced into a suitable host cell, such as Escherichia coli or Chinese hamster ovary (CHO) cells, which express the recombinant protein. The protein is subsequently purified using techniques such as affinity chromatography, ensuring high purity and yield.
Recombinant immunoglobulin heavy chain constant region gamma 2a is widely used in immunological research. Some of its key applications include:
- Antibody Engineering: Researchers use the recombinant IgG2a constant region to design and produce monoclonal antibodies with specific properties, such as enhanced binding affinity or altered effector functions.
- Therapeutic Development: The recombinant IgG2a constant region serves as a model for developing therapeutic antibodies targeting various diseases, including cancer and autoimmune disorders.
- Immune Response Studies: By using recombinant IgG2a in mouse models, scientists can investigate the mechanisms of immune responses and the role of different antibody isotypes in disease progression and protection.
