IGFBP3 Human, His

Insulin Like Growth Factor Binding Protein-3 Human Recombinant, His Tag
Cat. No.
BT16736
Source
Escherichia Coli.
Synonyms
Insulin-like growth factor-binding protein 3, Insulin Like Growth Factor Binding Protein-3, His Tag, IGFBP3, IBP-3, IGF-binding protein 3, IGFBP-3, IBP3, BP-53, Insulin-like growth factor binding protein 3 isoform b precursor.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

IGFBP3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 285 amino acids (28-291a.a.) and having a molecular mass of 31kDa.
IGFBP3 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
IGFBP3, a member of the insulin-like growth factor binding protein (IGFBP) family, encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. This protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II, circulating in the plasma to prolong the half-life of IGFs and alter their interaction with cell surface receptors. Characterizations have been made of alternate transcriptional splice variants that encode different isoforms.
Description
Recombinant Human IGFBP3, produced in E. coli, is a single, non-glycosylated polypeptide chain containing 285 amino acids (28-291a.a.) with a molecular mass of 31kDa. It is fused to a 21 amino acid His-tag at the N-terminus and purified by proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
IGFBP3 protein solution (1mg/ml) in 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For longer storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms
Insulin-like growth factor-binding protein 3, Insulin Like Growth Factor Binding Protein-3, His Tag, IGFBP3, IBP-3, IGF-binding protein 3, IGFBP-3, IBP3, BP-53, Insulin-like growth factor binding protein 3 isoform b precursor.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGASSAGLGP VVRCEPCDAR ALAQCAPPPA VCAELVREPG CGCCLTCALS EGQPCGIYTE RCGSGLRCQP SPDEARPLQA LLDGRGLCVN ASAVSRLRAY LLPAPPAPGN ASESEEDRSA GSVESPSVSS THRVSDPKFH PLHSKIIIIK KGHAKDSQRY KVDYESQSTD TQNFSSESKR ETEYGPCRRE MEDTLNHLKF LNVLSPRGVH IPNCDKKGFY KKKQCRPSKG RKRGFCWCVD KYGQPLPGYT TKGKEDVHCY SMQSK.

Product Science Overview

Introduction

Insulin-like Growth Factor Binding Protein-3 (IGFBP-3) is a crucial protein in the regulation of insulin-like growth factors (IGFs). It is the most abundant IGF binding protein in human serum and plays a significant role in cellular growth, differentiation, and proliferation . The recombinant form of IGFBP-3, tagged with a His-tag, is widely used in research to study its biological functions and interactions.

Structure and Expression

IGFBP-3 is a protein encoded by the IGFBP3 gene located on chromosome 7 in humans . The protein consists of 285 amino acids and has a molecular mass of approximately 31 kDa . The His-tag, a sequence of histidine residues, is added to the N-terminus of the protein to facilitate purification and detection .

Biological Functions

IGFBP-3 has both IGF-dependent and IGF-independent functions. It binds to IGF-I and IGF-II with high affinity, prolonging their half-life and modulating their interaction with cell surface receptors . This binding can either inhibit or stimulate the growth-promoting effects of IGFs on cell culture . Additionally, IGFBP-3 exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor, TMEM219/IGFBP-3R .

Mechanisms of Action

IGFBP-3 can act through several mechanisms:

  1. IGF-Dependent Mechanism: By binding to IGFs, IGFBP-3 regulates their availability and interaction with IGF receptors on the cell surface, thereby influencing cell growth and metabolism .
  2. IGF-Independent Mechanism: IGFBP-3 can induce apoptosis and inhibit cell proliferation independently of IGFs. This is mediated through its interaction with specific receptors such as TMEM219/IGFBP-3R .
Clinical Significance

IGFBP-3 is involved in various physiological and pathological processes. It has been implicated in growth regulation, cancer, and metabolic diseases. For instance, IGFBP-3 levels are often altered in cancer patients, and it has been studied as a potential biomarker for cancer diagnosis and prognosis .

Recombinant IGFBP-3 (His Tag)

The recombinant form of IGFBP-3, tagged with a His-tag, is produced in E. coli and purified using chromatographic techniques . This form is used in various research applications to study the protein’s structure, function, and interactions. The His-tag facilitates easy purification and detection, making it a valuable tool for researchers .

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Cat. No.
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Source
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THE BIOTEK
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