ANXA2 Human
Annexin A2 Human
Cat. No.
BT20246
Source
Human Adipose Tissue.
Synonyms
ANX2, ANX2L4, CAL1H, LIP2, LPC2, LPC2D, P36, PAP-IV, ANXA2, Annexin A2, Annexin-2, Annexin II, Lipocortin II, Calpactin-1 heavy chain, Calpactin I heavy chain, Chromobindin-8, p36, Protein I, Placental anticoagulant protein IV.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
The Human Annexin A2 produced from Human Adipose Tissue has a molecular mass of 38.472kDa (calculated without glycosylation) containing 338 amino acid residues.
Product Specs
Introduction
Annexin A2 (ANXA2) belongs to the annexin protein family and plays a crucial role in regulating cell growth and signal transduction pathways. This protein acts as an autocrine factor, promoting the formation of osteoclasts and bone resorption. Notably, ANXA2 has been linked to osteonecrosis in individuals with sickle cell disease. Furthermore, studies suggest that a decrease in ANXA2 expression is associated with the spread of osteosarcoma. Functioning as a component of the cell surface vitamin D binding protein complex, ANXA2 contributes to mediating the chemotactic cofactor effect. This protein is also implicated in dysferlin deficiency and various muscular dystrophies. Additionally, research indicates that an increase in ANXA2 expression is associated with the differentiation of human colon adenocarcinoma cells.
Description
Derived from human adipose tissue, this recombinant Human Annexin A2 protein exhibits a molecular weight of 38.472 kDa (excluding glycosylation). It comprises 338 amino acid residues.
Physical Appearance
White powder, lyophilized (freeze-dried) and filtered.
Formulation
Following filtration through a 0.4 μm filter, the ANXA2 protein undergoes lyophilization in a buffer solution composed of 0.05M phosphate, 0.075M NaCl, at a pH of 6.5. The final protein concentration is 0.5 mg/ml.
Solubility
To generate a working stock solution of approximately 0.5 mg/ml, reconstitute the lyophilized pellet by adding deionized water and ensure complete dissolution. Note: This product is not sterile. Prior to use in cell culture, sterile filtration is mandatory.
Stability
Lyophilized protein should be stored at -20°C. After reconstitution, aliquot the product to minimize repeated freeze-thaw cycles. The reconstituted protein demonstrates stability at 4°C for a limited duration and remains stable for up to two weeks at this temperature.
Purity
Purity levels exceed 90.0%, as determined by SDS-PAGE analysis.
Synonyms
ANX2, ANX2L4, CAL1H, LIP2, LPC2, LPC2D, P36, PAP-IV, ANXA2, Annexin A2, Annexin-2, Annexin II, Lipocortin II, Calpactin-1 heavy chain, Calpactin I heavy chain, Chromobindin-8, p36, Protein I, Placental anticoagulant protein IV.
Source
Human Adipose Tissue.
Amino Acid Sequence
STVHEILCKL SLEGDHSTPP SAYGSVKAYT NFDAERDALN IETAIKTKGV DEVTIVNILT NRSNAQRQDI AFAYQRRTKK ELASALKSAL SGHLETVILG LLKTPAQYDA SELKASMKGL GTDEDSLIEI ICSRTNQELQ EINRVYKEMY KTDLEKDIIS DTSGDFRKLM VALAKGRRAE DGSVIDYELI DQDARDLYDA GVKRKGTDVP KWISIMTERS VPHLQKVFDR YKSYSPYDML ESIRKEVKGD LENAFLNLVQ CIQNKPLYFA DRLYDSMKGK GTRDKVLIRI MVSRSEVDML KIRSEFKRKY GKSLYYYIQQ DTKGDYQKAL LYLCGGDD.
Product Science Overview
Gene and Structure
The ANXA2 gene is located on chromosome 15q22.2 and has three pseudogenes on chromosomes 4, 9, and 10 . Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene . Annexin A2 can exist as a monomer or as part of a heterotetramer complex, often interacting with other proteins such as S100A10 .
Functions
Annexin A2 is involved in several key cellular functions:
- Cell Motility: It plays a significant role in the movement of epithelial cells.
- Membrane-Associated Protein Complexes: It helps link these complexes to the actin cytoskeleton.
- Endocytosis: Annexin A2 is involved in the internalization of substances into the cell.
- Fibrinolysis: It acts as a receptor for tissue plasminogen activator (tPA) on the cell surface, facilitating plasmin generation and thus playing a role in the breakdown of blood clots .
- Signal Transduction: It is involved in the regulation of cellular growth and signal transduction pathways .
Clinical Significance
Annexin A2 has been implicated in various pathological conditions:
- Cancer: Overexpression of ANXA2 has been reported in several types of tumors, including hepatocellular carcinoma and breast cancer .
- Brain Tumors: Epigenetic regulation of Annexin A2 is a key determinant of mesenchymal transformation in brain tumors .
- Preeclampsia: Maternal deficiency of the ANXA2 gene contributes to shallow decidual invasion by placental cytotrophoblast cells, highlighting its role in the pathogenesis of severe preeclampsia .
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