HTATIP2 Antibody
HIV-1 Tat Interactive Protein 2, Mouse Anti Human
Cat. No.
BT19815
Source
Synonyms
TIP30, CC3, SDR44U1, HTATIP2, EC=1.1.1.-, HIV-1 TAT-interactive protein 2, FLJ26963.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
HTATIP2, a member of the short-chain dehydrogenases/reductases (SDR) family, functions as a tumor suppressor. It achieves this by suppressing metabolism, inhibiting angiogenesis, and inducing the expression of apoptosis-related genes Bad and Siva. HTATIP2 interacts with the activation domain of HIV-1 TAT, enhancing its transcription through phosphorylation of RNA polymerase II (Pol II). Defects in HTATIP2 are linked to hepatocellular carcinomas and apoptotic-resistant tumor cells, suggesting its potential in antitumor therapy.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The solution contains 1mg/ml of HTATIP2 antibody in a buffer consisting of PBS at pH 7.4, 10% glycerol, and 0.02% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), keep at 4°C. For longer storage periods, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The product is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This HTATIP2 antibody has been validated for use in ELISA, Western blot analysis, Flow cytometry, and ICC/IF to ensure its specificity and reactivity. However, optimal working dilutions should be determined empirically for each application.
Synonyms
TIP30, CC3, SDR44U1, HTATIP2, EC=1.1.1.-, HIV-1 TAT-interactive protein 2, FLJ26963.
Purification Method
HTATIP2 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT5D6AT.
Immunogen
Anti-human HTATIP2 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human HTATIP2 amino acids 1-242 purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and ? light chain.
Product Science Overview
Introduction
Gene and Protein Structure
HTATIP2 is a protein-coding gene located on chromosome 11 in humans. The protein itself is approximately 30 kDa in size and belongs to the short-chain dehydrogenases/reductases (SDR) family . It has multiple isoforms, with Isoform 1 acting as a metastasis suppressor and Isoform 2 having antiapoptotic effects .
Function and Mechanism
HTATIP2 is involved in several critical cellular functions:
- Tumor Suppression: HTATIP2 acts as a tumor suppressor by inhibiting nuclear import and competing with nuclear import substrates for binding to nuclear transport receptors . It also induces the expression of apoptosis-related genes such as Bad and Siva .
- Apoptosis and Angiogenesis: The protein plays a role in the positive regulation of programmed cell death and transcription by RNA polymerase II. It also has antiangiogenic properties, which help in inhibiting the formation of new blood vessels in tumors .
- Redox Sensor: HTATIP2 may act as a redox sensor linked to transcription through the regulation of nuclear import .
Clinical Significance
Mouse Anti-Human HTATIP2 Antibody
The mouse anti-human HTATIP2 monoclonal antibody is used in research to study the protein’s function and its role in various diseases. This antibody is derived from hybridization of mouse myeloma cells with spleen cells from BALB/c mice immunized with recombinant human HTATIP2 . It is used in various applications, including ELISA, Western Blot, Flow Cytometry, Immunocytochemistry, and Immunofluorescence .
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