HSP90B1 Antibody

Heat Shock Protein 90kDa Beta (GRP94) Member 1, Mouse Anti Human
Cat. No.
BT11576
Source
Synonyms
ECGP, GP96, TRA1, GRP94, HSP90B1, Endoplasmin, Heat shock protein 90 kDa beta member 1, 94 kDa glucose-regulated protein, gp96 homolog, Tumor rejection antigen 1.
Appearance
Sterile Filtered clear solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
HSP90B1 is an abundant molecular chaperone residing in the endoplasmic reticulum (ER) lumen. As a stress protein and part of the Hsp90 family, HSP90B1 maintains protein homeostasis within the secretory pathway. It also plays a role in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway in antigen-presenting cells. HSP90B1 is crucial for signal transduction, protein folding and degradation, and morphological evolution. This protein interacts with numerous cochaperones and participates in the folding of newly synthesized proteins and the stabilization and refolding of denatured proteins following stress. Notably, HSP90B1 exhibits high expression levels in human gastric carcinoma BGC-823 cells throughout the entire cell cycle.
Physical Appearance
Clear solution, sterile filtered.
Formulation
1 mg/ml in PBS, pH 7.4, with 0.1% Sodium Azide.
Storage Procedures
Store at 4°C for up to 1 month. For extended storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
Stable for 12 months at -20°C and 1 month at 4°C.
Applications
HSP90B1 antibody has undergone ELISA and Western blot analysis to ensure its specificity and reactivity. However, optimal working dilutions should be determined empirically for each application. For immunofluorescence analysis, a dilution range of 1:100 to 1:200 is recommended, with a starting dilution of 1:100.
Synonyms
ECGP, GP96, TRA1, GRP94, HSP90B1, Endoplasmin, Heat shock protein 90 kDa beta member 1, 94 kDa glucose-regulated protein, gp96 homolog, Tumor rejection antigen 1.
Purification Method
HSP90B1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
P2H3AT.
Immunogen
Anti-human HSP90B1 mAb, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human HSP90B1 amino acids 676-803 purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and κ light chain.

Product Science Overview

Introduction

Heat Shock Protein 90kDa Beta (GRP94), also known as HSP90B1, is a molecular chaperone that plays a critical role in the folding, assembly, and stabilization of a wide range of proteins. It is a member of the HSP90 family and is predominantly found in the endoplasmic reticulum (ER). GRP94 is also referred to as endoplasmin, gp96, or ERp99 .

Structure and Function

GRP94 is an ATP-binding protein that assists in the proper folding of newly synthesized proteins and the refolding of misfolded proteins within the ER. It is involved in the processing and transport of secreted proteins and is essential for the proper functioning of the immune system. GRP94 interacts with Toll-like receptors (TLRs) and integrins, playing a crucial role in both innate and adaptive immunity .

Biological Significance

GRP94 is implicated in various cellular processes, including:

  • Protein Folding: Assists in the folding of proteins within the ER.
  • Stress Response: Plays a role in the cellular response to stress, particularly ER stress.
  • Immune Regulation: Essential for the proper functioning of the immune system by regulating TLRs and integrins.
  • Calcium Homeostasis: Involved in the regulation of calcium ion binding within the ER .
Mouse Anti Human GRP94 Antibody

Monoclonal antibodies against GRP94, such as those produced in mice, are valuable tools in research and diagnostics. These antibodies are used to detect and quantify GRP94 in various biological samples. They are also employed in studying the role of GRP94 in different diseases and in the development of therapeutic interventions .

Applications in Research

The mouse anti-human GRP94 antibody has several applications, including:

  • Western Blotting: Used to detect GRP94 protein levels in cell and tissue lysates.
  • Immunohistochemistry: Helps in visualizing the localization of GRP94 in tissue sections.
  • Flow Cytometry: Utilized to analyze the expression of GRP94 on the cell surface.
  • Immunoprecipitation: Employed to isolate GRP94 from complex protein mixtures for further analysis .

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