HSD17B10 Human
Hydroxysteroid (17-beta) Dehydrogenase 10 Human Recombinant
Cat. No.
BT9996
Source
Escherichia Coli.
Synonyms
17b-HSD10, ABAD, CAMR, DUPXp11.22, ERAB, HADH2, HCD2, MHBD, MRPP2, MRX17, MRX31, SCHAD, MRXS10, SDR5C1.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
HSD17B10 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 271 amino acids (12-261 a.a.) and having a molecular mass of 28.1 kDa. HSD17B10 protein is fused to a 21 amino acid His-Tag at N-terminus and purified by standard chromatography.
Product Specs
Introduction
HSD17B10, a component of mitochondrial ribonuclease P, plays a crucial role in the maturation of mitochondrial tRNA. This enzyme, composed of MRPP1/RG9MTD1, MRPP2/HSD17B10, and MRPP3/KIAA0391, is responsible for cleaving tRNA molecules at their 5' ends. Notably, HSD17B10 interacts with intracellular amyloid-beta, contributing to the neuronal dysfunction observed in Alzheimer's disease.
Description
Recombinant HSD17B10, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 271 amino acids (residues 12-261). With a molecular weight of 28.1 kDa, this protein is produced with a 21-amino acid His-Tag fused at the N-terminus to facilitate purification via standard chromatography techniques.
Physical Appearance
The product is a sterile, colorless solution that has been filtered for purity.
Formulation
The HSD17B10 protein is provided at a concentration of 1 mg/ml in a buffer solution containing 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 1 mM DTT, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage periods, it is recommended to freeze the product at -20°C. To further enhance stability during long-term storage, the addition of a carrier protein like HSA or BSA (0.1%) is advisable. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the HSD17B10 protein is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
17b-HSD10, ABAD, CAMR, DUPXp11.22, ERAB, HADH2, HCD2, MHBD, MRPP2, MRX17, MRX31, SCHAD, MRXS10, SDR5C1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MVAVITGGAS GLGLATAERL VGQGASAVLL DLPNSGGEAQ AKKLGNNCVF APADVTSEKD VQTALALAKG KFGRVDVAVN CAGIAVASKT YNLKKGQTHT LEDFQRVLDV NLMGTFNVIR LVAGEMGQNE PDQGGQRGVI INTASVAAFE GQVGQAAYSA SKGGIVGMTL PIARDLAPIG IRVMTIAPGL FGTPLLTSLP EKVCNFLASQ VPFPSRLGDP AEYAHLVQAI IENPFLNGEV IRLDGAIRMQ P.
Product Science Overview
Function and Role in Metabolism
HSD17B10 is involved in the oxidation of a wide variety of substrates, including fatty acids, alcohols, and steroids . It is particularly important in the metabolism of neurosteroids, which are critical for brain function and development . The enzyme also participates in mitochondrial tRNA maturation, which is essential for protein synthesis within mitochondria .
Clinical Significance
Mutations in the HSD17B10 gene can lead to 17β-Hydroxysteroid Dehydrogenase Type 10 (HSD10) deficiency, a rare metabolic disorder characterized by developmental delay, intellectual disability, and other neurological symptoms . Additionally, HSD17B10 has been implicated in the development of Alzheimer’s disease due to its interaction with amyloid-beta peptides .
Research and Applications
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